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Open Access Research article

Identifying intrinsic and extrinsic determinants that regulate internal initiation of translation mediated by the FMR1 5' leader

Tara Dobson2, Erika Kube1, Stephanie Timmerman2 and Les A Krushel123*

Author Affiliations

1 Department of Pharmacology, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA

2 Department of Biochemistry and Molecular Genetics, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA

3 Neurosciences Program, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA

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BMC Molecular Biology 2008, 9:89  doi:10.1186/1471-2199-9-89

Published: 15 October 2008

Additional files

Additional file 1:

Different FMRP antibodies recognize similar proteins in hippocampal extract and C6 cells. Western blots of lysates from rat brain hippocampus and C6 cells were probed with a mouse monoclonal (7G1, Developmental Hybridoma Bank) and a rabbit polyclonal (sc-28739, Santa Cruz Biotechnology) antibody, both of which are directed against FMRP. The two antibodies mainly recognize a single band of approximately 80 kD.

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