Normalisation genes for expression analyses in the brown alga model Ectocarpus siliculosus

doi:10.1186/1471-2199-9-75

BMC Molecular Biology

Volume 9

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Le Bail A
Dittami SM
de Franco PO
Rousvoal S
Cock MJ
Tonon T
Charrier B

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Dittami SM
de Franco PO
Rousvoal S
Cock MJ
Tonon T
Charrier B
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Methodology article

Normalisation genes for expression analyses in the brown alga model Ectocarpus siliculosus

Aude Le Bail¹^,2^* , Simon M Dittami¹^,2^* , Pierre-Olivier de Franco¹^,2 , Sylvie Rousvoal¹^,2 , Mark J Cock¹^,2 , Thierry Tonon¹^,2 and Bénédicte Charrier¹^,2

¹UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, Station Biologique, F 29682, Roscoff, France

²CNRS, UMR 7139 Végétaux marins et Biomolécules, Station Biologique, F 29682, Roscoff, France

author email corresponding author email* Contributed equally

BMC Molecular Biology 2008, 9:75doi:10.1186/1471-2199-9-75


Published:	18 August 2008

Additional files

Additional file 1:

One-way ANOVA test for the significance of the biological triplicate averaging. An ANOVA was performed on the three biological replicates of all the data, with the groups corresponding to the different treatments. The resulting p-values are shown in the table.

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Additional file 2:

Gene expression during the diurnal cycle.

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Additional file 3:

Quantification and quality of the RNAs used in this study.

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Additional file 4:

RNA extracts considered in this study. From the 83 RNAs run on the gel, the ones used for the cDNA synthesis are labelled in red. Between 400 to 900 ng of RNA were loaded on the gel.

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