Table 2

GlaI activity on methylated oligonucleotide duplexes

Oligonucleotide duplex
The central part of structure of a duplex
Relative enzyme's activity (%) and standard error

G7*/G8
5'-TTTTCGMGMTGACG-3'
100 ± 5

3'-AAAAGMGMGACTGC-5'

G8*/G7
5'-CGTCAGMGMGAAAA-3'
97 ± 5

3'-GCAGTMGMGCTTTT-5'

G3*/G8
5'-TTTTCGMGCTGACG-3'
28 ± 2

3'-AAAAGMGMGACTGC-5'

G8*/G3
5'-CGTCAGMGMGAAAA-3'
40 ± 2

3'-GCAGTCGMGCTTTT-5'

G4*/G7
5'-CGTCAGMGCGAAAA-3'
31 ± 2

3'-GCAGTMGMGCTTTT-5'

G7*/G4
5'-TTTTCGMGMTGACG-3'
36 ± 2

3'-AAAAGCGMGACTGC-5'

G11*/G12
5'-TTTTCAMGMTGACG-3'
61 ± 3

3'-AAAAGTGMGACTGC-5'

G12*/G11
5'-CGTCAGMGTGAAAA-3'
60 ± 3

3'-GCAGTMGMACTTTT-5'

G6*/G7
5'-CGTCAGCGMGAAAA-3'
16 ± 1

3'-GCAGTMGMGCTTTT-5'

G7*/G6
5'-TTTTCGMGMTGACG-3'
17 ± 1

3'-AAAAGMGCGACTGC-5'

G5*/G8
5'-TTTTCGCGMTGACG-3'
17 ± 1

3'-AAAAGMGMGACTGC-5'

G8*/G5
5'-CGTCAGMGMGAAAA-3'
17 ± 1

3'-GCAGTMGCGCTTTT-5'

G13*/G14
5'-TTTTCGTGMTGACG-3'
21 ± 1

3'-AAAAGMAMGACTGC-5'

G14*/G13
5'-CGTCAGMAMGAAAA-3'
16 ± 1

3'-GCAGTMGTGCTTTT-5'

G20*/G21
5'-TTTTCGMAMTGACG-3'
17 ± 1

3'-AAAAGMGTGACTGC-5'

G21*/G20
5'-CGTCAGTGMGAAAA-3'
25 ± 1

3'-GCAGTMAMGCTTTT-5'

G3*/G4
5'-TTTTCGMGCTGACG-3'
5 ± 1

3'-AAAAGCGMGACTGC-5'

G4*/G3
5'-CGTCAGMGCGAAAA-3'
6 ± 1

3'-GCAGTCGMGCTTTT-5'

G12*/G17
5'-CGTCAGMGTGAAAA-3'
28 ± 1

3'-GCAGTCGMACTTTT-5'

G17*/G12
5'-TTTTCAMGCTGACG-3'
13 ± 1

3'-AAAAGTGMGACTGC-5'

G15*/G16
5'-TTTTCAMGTTGACG-3'
26 ± 1

3'-AAAAGTGMAACTGC-5'

G16*/G15
5'-CGTCAAMGTGAAAA-3'
23 ± 1

3'-GCAGTTGMACTTTT-5'

G18*/G19
5'-TTTTCGMGTTGACG-3'
12 ± 1

3'-AAAAGCGMAACTGC-5'

G19*/G18
5'-CGTCAAMGCGAAAA-3'
14 ± 1

3'-GCAGTTGMGCTTTT-5'

G7*/G2
5'-TTTTCGMGMTGACG-3'
< 1

3'-AAAAGCGCGACTGC-5'

G2*/G7
5'-CGTCAGCGCGAAAA-3'
< 1

3'-GCAGTMGMGCTTTT-5'

G13*/G35
5'-TTTTCGTGMTGACG-3'
< 1

3'-AAAAGMACGACTGC-5'

G35*/G13
5'-CGTCAGCAMGAAAA-3'
< 1

3'-GCAGTMGTGCTTTT-5'

G5*/G6
5'-TTTTCGCGMTGACG-3'
< 1

3'-AAAAGMGCGACTGC-5'

G6*/G5
5'-CGTCAGCGMGAAAA-3'
< 1

3'-GCAGTMGCGCTTTT-5'

G12*/G32
5'-CGTCAGMGTGAAAA-3'
< 1

3'-GCAGTMGCACTTTT-5'

G32*/G12
5'-TTTTCACGMTGACG-3'
< 1

3'-AAAAGTGMGACTGC-5'

G33*/G34
5'-TTTTCATGMTGACG-3'
< 1

3'-AAAAGTAMGACTGC-5'

G34*/G3
5'-CGTCAGMATGAAAA-3'
< 1

3'-GCAGTMGTACTTTT-5'

G13*/36
5'-TTTTCGTGMTGACG-3'
< 1

3'-AAAAGCAMGACTGC-5'

G36*/13
5'-CGTCAGMACGAAAA-3'
< 1

3'-GCAGTMGTGCTTTT-5'

G3*/G6
5'-TTTTCGMGCTGACG-3'
< 1

3'-AAAAGMGCGACTGC-5'

G6*/G3
5'-CGTCAGCGMGAAAA-3'
< 1

3'-GCAGTCGMGCTTTT-5'

G4*/G5
5'-CGTCAGMGCGAAAA-3'
< 1

3'-GCAGTMGCGCTTTT-5'

G5*/G4
5'-TTTTCGCGMTGACG-3'
< 1

3'-AAAAGCGMGACTGC-5'

G22*/G23
5'-TTTTCMMGMTGACG-3'
< 1

3'-AAAAGGGMGACTGC-5'

G23*/G22
5'-CGTCAGMGGGAAAA-3'
< 1

3'-GCAGTMGMMCTTTT-5'

G24*/G25
5'-TTTTCGGGMTGACG-3'
< 1

3'-AAAAGMMMGACTGC-5'

G25*/G24
5'-CGTCAGMMMGAAAA-3'
< 1

3'-GCAGTMGGGCTTTT-5'

G28*/G29
5'-TTTTCTMGMTGACG-3'
< 1

3'-AAAAGAGMGACTGC-5'

G29*/G28
5'-CGTCAGMGAGAAAA-3'
< 1

3'-GCAGTMGMTCTTTT-5'

G30*/G31
5'-TTTTCGAGMTGACG-3'
< 1

3'-AAAAGMTMGACTGC-5'

G31*/G30
5'-CGTCAGMTMGAAAA-3'
< 1

3'-GCAGTMGAGCTTTT-5'

G37*/G38
5'-TTTTCTMGATGACG-3'
< 1

3'-AAAAGAGMTACTGC-5'

G38*/G37
5'-CGTCATMGAGAAAA-3'
< 1

3'-GCAGTAGMTCTTTT-5'

G26*/G23
5'-TTTTCCMGMTGACG-3'
< 1

3'-AAAAGGGMGACTGC-5'

G23*/G26
5'-CGTCAGMGGGAAAA-3'
< 1

3'-GCAGTMGMCCTTTT-5'

G27*/G24
5'-CGTCAGMCMGAAAA-3'
< 1

3'-GCAGTMGGGCTTTT-5'

G24*/G27
5'-TTTTCGGGMTGACG-3'
< 1

3'-AAAAGMCMGACTGC-5'


Tarasova et al. BMC Molecular Biology 2008 9:7   doi:10.1186/1471-2199-9-7