Table 2

GlaI activity on methylated oligonucleotide duplexes

Oligonucleotide duplex

The central part of structure of a duplex

Relative enzyme's activity (%) and standard error

G7*/G8

5'-TTTTCGMGMTGACG-3'

100 ± 5

3'-AAAAGMGMGACTGC-5'

G8*/G7

5'-CGTCAGMGMGAAAA-3'

97 ± 5

3'-GCAGTMGMGCTTTT-5'

G3*/G8

5'-TTTTCGMGCTGACG-3'

28 ± 2

3'-AAAAGMGMGACTGC-5'

G8*/G3

5'-CGTCAGMGMGAAAA-3'

40 ± 2

3'-GCAGTCGMGCTTTT-5'

G4*/G7

5'-CGTCAGMGCGAAAA-3'

31 ± 2

3'-GCAGTMGMGCTTTT-5'

G7*/G4

5'-TTTTCGMGMTGACG-3'

36 ± 2

3'-AAAAGCGMGACTGC-5'

G11*/G12

5'-TTTTCAMGMTGACG-3'

61 ± 3

3'-AAAAGTGMGACTGC-5'

G12*/G11

5'-CGTCAGMGTGAAAA-3'

60 ± 3

3'-GCAGTMGMACTTTT-5'

G6*/G7

5'-CGTCAGCGMGAAAA-3'

16 ± 1

3'-GCAGTMGMGCTTTT-5'

G7*/G6

5'-TTTTCGMGMTGACG-3'

17 ± 1

3'-AAAAGMGCGACTGC-5'

G5*/G8

5'-TTTTCGCGMTGACG-3'

17 ± 1

3'-AAAAGMGMGACTGC-5'

G8*/G5

5'-CGTCAGMGMGAAAA-3'

17 ± 1

3'-GCAGTMGCGCTTTT-5'

G13*/G14

5'-TTTTCGTGMTGACG-3'

21 ± 1

3'-AAAAGMAMGACTGC-5'

G14*/G13

5'-CGTCAGMAMGAAAA-3'

16 ± 1

3'-GCAGTMGTGCTTTT-5'

G20*/G21

5'-TTTTCGMAMTGACG-3'

17 ± 1

3'-AAAAGMGTGACTGC-5'

G21*/G20

5'-CGTCAGTGMGAAAA-3'

25 ± 1

3'-GCAGTMAMGCTTTT-5'

G3*/G4

5'-TTTTCGMGCTGACG-3'

5 ± 1

3'-AAAAGCGMGACTGC-5'

G4*/G3

5'-CGTCAGMGCGAAAA-3'

6 ± 1

3'-GCAGTCGMGCTTTT-5'

G12*/G17

5'-CGTCAGMGTGAAAA-3'

28 ± 1

3'-GCAGTCGMACTTTT-5'

G17*/G12

5'-TTTTCAMGCTGACG-3'

13 ± 1

3'-AAAAGTGMGACTGC-5'

G15*/G16

5'-TTTTCAMGTTGACG-3'

26 ± 1

3'-AAAAGTGMAACTGC-5'

G16*/G15

5'-CGTCAAMGTGAAAA-3'

23 ± 1

3'-GCAGTTGMACTTTT-5'

G18*/G19

5'-TTTTCGMGTTGACG-3'

12 ± 1

3'-AAAAGCGMAACTGC-5'

G19*/G18

5'-CGTCAAMGCGAAAA-3'

14 ± 1

3'-GCAGTTGMGCTTTT-5'

G7*/G2

5'-TTTTCGMGMTGACG-3'

< 1

3'-AAAAGCGCGACTGC-5'

G2*/G7

5'-CGTCAGCGCGAAAA-3'

< 1

3'-GCAGTMGMGCTTTT-5'

G13*/G35

5'-TTTTCGTGMTGACG-3'

< 1

3'-AAAAGMACGACTGC-5'

G35*/G13

5'-CGTCAGCAMGAAAA-3'

< 1

3'-GCAGTMGTGCTTTT-5'

G5*/G6

5'-TTTTCGCGMTGACG-3'

< 1

3'-AAAAGMGCGACTGC-5'

G6*/G5

5'-CGTCAGCGMGAAAA-3'

< 1

3'-GCAGTMGCGCTTTT-5'

G12*/G32

5'-CGTCAGMGTGAAAA-3'

< 1

3'-GCAGTMGCACTTTT-5'

G32*/G12

5'-TTTTCACGMTGACG-3'

< 1

3'-AAAAGTGMGACTGC-5'

G33*/G34

5'-TTTTCATGMTGACG-3'

< 1

3'-AAAAGTAMGACTGC-5'

G34*/G3

5'-CGTCAGMATGAAAA-3'

< 1

3'-GCAGTMGTACTTTT-5'

G13*/36

5'-TTTTCGTGMTGACG-3'

< 1

3'-AAAAGCAMGACTGC-5'

G36*/13

5'-CGTCAGMACGAAAA-3'

< 1

3'-GCAGTMGTGCTTTT-5'

G3*/G6

5'-TTTTCGMGCTGACG-3'

< 1

3'-AAAAGMGCGACTGC-5'

G6*/G3

5'-CGTCAGCGMGAAAA-3'

< 1

3'-GCAGTCGMGCTTTT-5'

G4*/G5

5'-CGTCAGMGCGAAAA-3'

< 1

3'-GCAGTMGCGCTTTT-5'

G5*/G4

5'-TTTTCGCGMTGACG-3'

< 1

3'-AAAAGCGMGACTGC-5'

G22*/G23

5'-TTTTCMMGMTGACG-3'

< 1

3'-AAAAGGGMGACTGC-5'

G23*/G22

5'-CGTCAGMGGGAAAA-3'

< 1

3'-GCAGTMGMMCTTTT-5'

G24*/G25

5'-TTTTCGGGMTGACG-3'

< 1

3'-AAAAGMMMGACTGC-5'

G25*/G24

5'-CGTCAGMMMGAAAA-3'

< 1

3'-GCAGTMGGGCTTTT-5'

G28*/G29

5'-TTTTCTMGMTGACG-3'

< 1

3'-AAAAGAGMGACTGC-5'

G29*/G28

5'-CGTCAGMGAGAAAA-3'

< 1

3'-GCAGTMGMTCTTTT-5'

G30*/G31

5'-TTTTCGAGMTGACG-3'

< 1

3'-AAAAGMTMGACTGC-5'

G31*/G30

5'-CGTCAGMTMGAAAA-3'

< 1

3'-GCAGTMGAGCTTTT-5'

G37*/G38

5'-TTTTCTMGATGACG-3'

< 1

3'-AAAAGAGMTACTGC-5'

G38*/G37

5'-CGTCATMGAGAAAA-3'

< 1

3'-GCAGTAGMTCTTTT-5'

G26*/G23

5'-TTTTCCMGMTGACG-3'

< 1

3'-AAAAGGGMGACTGC-5'

G23*/G26

5'-CGTCAGMGGGAAAA-3'

< 1

3'-GCAGTMGMCCTTTT-5'

G27*/G24

5'-CGTCAGMCMGAAAA-3'

< 1

3'-GCAGTMGGGCTTTT-5'

G24*/G27

5'-TTTTCGGGMTGACG-3'

< 1

3'-AAAAGMCMGACTGC-5'

Tarasova et al. BMC Molecular Biology 2008 9:7   doi:10.1186/1471-2199-9-7