PSMB2 and RPL32 are suitable denominators to normalize gene expression profiles in bronchoalveolar cells

doi:10.1186/1471-2199-9-69

BMC Molecular Biology

Volume 9

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Kriegova E
Arakelyan A
Fillerova R
Zatloukal J
Mrazek F
Navratilova Z
Kolek V
du Bois RM
Petrek M

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Arakelyan A
Fillerova R
Zatloukal J
Mrazek F
Navratilova Z
Kolek V
du Bois RM
Petrek M
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Research article

PSMB2 and RPL32 are suitable denominators to normalize gene expression profiles in bronchoalveolar cells

Eva Kriegova¹ , Arsen Arakelyan¹ , Regina Fillerova¹ , Jaromir Zatloukal² , Frantisek Mrazek¹ , Zdenka Navratilova¹ , Vitezslav Kolek² , Roland M du Bois³ and Martin Petrek¹

¹Department of Immunology, Palacky University, The Czech Republic

²Department of Respiratory Medicine, Palacky University & Faculty Hospital, Olomouc, The Czech Republic

³Interstitial Lung Disease Unit, Royal Brompton Hospital, London, UK

author email corresponding author email

BMC Molecular Biology 2008, 9:69doi:10.1186/1471-2199-9-69


Published:	31 July 2008

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Description of used statistical approaches.

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Table E1. Clinical and laboratory characteristics of investigated subjects.

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Definition of terms.

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Figure E1. RNA quality assessment (a representative example) by 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA). This figure shows typical chromatogram of microcapillary electrophoresis of total RNA preparation of good quality extracted from bronchoalveolar lavage cells. Electropherogram shows 18S and 28S rRNA peaks. FU – Fluorescence units.

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Figure E2. Expression levels of ten housekeeping genes in bronchoalveolar cells from sarcoidosis patients and normal subjects from the 2nd cohort. Expression levels of ten housekeeping genes in CTt values in bronchoalveolar cells from sarcoidosis patients (n = 63) a normal subjects (n = 17). The data are presented as means (columns) ± SD (errorbars). White columns represent the control group, dark columns sarcoidosis patients.

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