|
Primer sequences and optimum PCR conditions for amplifying the products |
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| Gene |
Forward (5' to 3') Reverse (5' to 3') |
Product size (bp) |
Primer Used (nM) |
Annealing Temp (T°) |
|
|
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| Actb |
TCCGCCGCCGGCCCACACT AGTCCTTCTGGCCCATGC |
188 |
0.40 0.40 |
60 |
| Gapdh |
CAAGTTCCACGGCACGGTCA CTCGGCACCAGCATCACCC |
118 |
0.40 0.40 |
68 |
| Hprt1 |
ACGTCGAGGACTTGGAAAGGGTGTT GGCCTCCCATCTCCTTCATCACATC |
96 |
0.40 0.40 |
68 |
| H2afz |
AGAGCCGGCTGCCAGTTCC CAGTCGCGCCCACACGTCC |
85 |
0.20 0.33 |
59 |
| Ubc |
GTGACACCATCGAGAAT GACACCTCCCCTCAGAC |
173 |
0.40 0.30 |
60 |
| Ppia |
TCCAGGGTTTATGTGCCAGGGTG CGTTTGCCATGGACAAGATGCC |
137 |
0.30 0.30 |
68 |
| Eef1e1 |
ACCGCAGAAGAGAAAGCCATAG AGCGATGTAGCCCATAGTAGAGGA |
190 |
0.50 0.50 |
68 |
| G6pdx |
AGCCCGCCTCCACTGACTCC ACCACGTTGTCCGCCTGCAC |
88 |
0.40 0.30 |
60 |
| Tbp |
GCTGAATATAATCCCAAGCGGTTTGC AAATCAGCGCTGTGGTTCGTGGCTCTC |
73 |
0.30 0.30 |
68 |
| Polr2a |
AGACTTCTCGGCCCGCACTG ACTTGGCGCCTGGGTACTGG |
176 |
0.40 0.40 |
68 |
| B2m |
GCTCCGTCTTGGGCTTG CGGATGAAACCCAGATACATAG |
152 |
0.33 0.33 |
60 |
| Pgk1 |
TGTTGGTCGGGCGAAGCAG CAGTGTCTCCACCGCCGATG |
149 |
0.50 0.20 |
60 |
| Ywhaz |
GGTCTGGCCCTTAACTTCTCTGTGTTCTA GCGTGCTGTCTTTGTATGATTCTTCACTT |
142 |
0.50 0.50 |
68 |
| POU5F1 |
CGAGTGAGAGGCAACTTGG CGGTTACAGAACCACACACG |
125 |
0.45 0.20 |
57 |
Mamo et al. BMC Molecular Biology 2008 9:67 doi:10.1186/1471-2199-9-67 |
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