Figure 1.

Renal, hepatic and adipose IR mRNA levels. Insulin receptor (IR) mRNA levels in the kidney (A, B), liver (C, D) and epididymal adipose tissue (E, F) of sham-treated rats (Control), rats treated with 1,25D₃ [150 IU/Kg (3.75 μg/Kg) one a day, for 15 days](1,25D₃), streptozotocin-induced diabetic rats (STZ) and streptozotocin-induced diabetic rats treated with 1,25D₃ (STZ+1,25D₃). On the right, the autoradiograph of a representative Northern blot experiment using the kidney (B), liver (D) and adipose tissue (F) of two rats. The sizes of the two major IR mRNA species are shown in the margin. On the left, densitometric analysis of three independent Northern blot experiments on kidney (A), liver (C), and adipose tissue (E). IR mRNA species were quantified separately, normalized to the respective 28S rRNA values and expressed per unit of RNA as a percentage of the values obtained in Control-rats (mean ± SEM). ^a p < 0.05 vs. Control-rats; ^b p < 0.05 vs. 1,25D₃-rats; and ^c p < 0.05 vs. STZ-rats.