Quantification of mRNA in single cells and modelling of RT-qPCR induced noise

Martin Bengtsson^1,2 , Martin Hemberg^3,5 , Patrik Rorsman¹ and Anders Ståhlberg^4,6

¹Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, The Churchill Hospital, Oxford, OX3 7LJ, UK

²Department of Clinical Sciences, Lund University, Clinical Research Centre, 205 02 Malmö, Sweden

³Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK

⁴Stem Cell Center, Lund University, BMC B10, 221 84 Lund, Sweden

⁵Department of Ophthalmology and Program in Neurobiology, Children's Hospital Boston, Harvard Medical School, 1 Blackfan Circle, Boston, MA 02115, USA

⁶Department of Clinical Neuroscience and Rehabilitation, Institute of Neurosciences and Physiology, Sahlgrenska Academy at Göteborg University, Medicinaregatan 9A, 413 90 Göteborg, Sweden