Degree of off-target knock down of CYLD and SOAT correlates with the concentration of the transfected siRNA. (a) Different concentrations of the siRNA directed against GFP were transfected in HeLa and (b) HEK cells. Expression of specific off-target genes (CYLD, SOAT), non-target genes used as negative control (LMNB1, C13ORF1) and genes unspecifically upregulated by antiviral cellular response (OAS1) were measured by qPCR 48 h post-transfection. Expression values were normalized to the average of 2 housekeeping genes (PGK, DCTN2). Correlation coefficients between the amount of siRNA transfected and the corresponding mRNA levels of the genes measured are given.
Tschuch et al. BMC Molecular Biology 2008 9:60 doi:10.1186/1471-2199-9-60