Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model

doi:10.1186/1471-2199-9-53

BMC Molecular Biology

Volume 9

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Research article

Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model

Kristina Langnaese¹ , Robin John¹ , Hannes Schweizer¹ , Uwe Ebmeyer² and Gerburg Keilhoff¹

¹Institute of Medical Neurobiology, University of Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany

²Department of Anaesthesiology and Critical Care Medicine, University of Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany

author email corresponding author email

BMC Molecular Biology 2008, 9:53doi:10.1186/1471-2199-9-53


Published:	28 May 2008

Additional files

Additional file 1:

Workflow schema for reference gene selection. The diagram illustrates the process of stable gene selection for normalization purposes in relative quantification. Workflow is given for two software programs, geNorm and NormFinder. The scheme is based on [11,19] as well as the accompanying software manuals [55,56]. For the principle of these programs see [11,19]. Transformation of raw Ct values into quantities can alternatively be performed using standard curves. The use of five to ten candidate reference genes is strongly recommended [56]. (HKG: Abbr. housekeeping gene).

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