Figure 7.

Developmental expression of S. exigua Tre-2 mRNA in the midgut (A) and fat body (B). [α-³²P] dCTP-labeled SeTre-2 cDNA was amplified using specific primers SeTreFP and SeTreRP and used as a probe. β-Actin was labeled with [α-³²P]dCTP as a control.(A) RNA was extracted from the midgut third instar (3L), fourth instar (4L), fifth instar (5L) and wandering (pre-pupae) larvae (W) and from pupae (P). (B) RNA was extracted from the fat body of fifth instar (5L) and wandering (pre-pupae) larvae (W) and pupae (P).