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Primers used to amplify target loci |
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| Targets |
Primer name |
Primer sequence |
|
|
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| Factor V |
M13F-FV Forward |
cacgacgttgtaaaacgac ttaattggttccagcgaaagc |
| M13R-FV Reverse |
ggataacaatttcacacagg tccaataccaacagacctgg |
|
| Reverse Trans. |
M13F-RT Forward |
cacgacgttgtaaaacgac taggacctacacctgtcaacataa |
| M13R-RT Reverse |
ggataacaatttcacacagg cttctgtatgtcattgacagtcca |
|
| RBR exon10-11 |
RB-1N1 Forward |
ggagcagtcagaataagcggaatg |
| RB-1N1 Reverse |
taaccaagcccacatcgacgtcatc |
|
| RB-1N2 Forward |
cacgacgttgtaaaacgac ggctacaattatctcagcagataag |
|
| RB-1N2 Reverse |
ggataacaatttcacacagg gtcctcttactttccggttgctgaa |
|
| RBR exon14-17 |
RB-2N1 Forward |
tgaagaagacgccctgtttggcatc |
| RB-2N1 Reverse |
gttgcttaagtgaagaagccgatct |
|
| RB-2N2 Forward |
cacgacgttgtaaaacgac tgagtgtcttggaaaatgattacga |
|
| RB-2N2 Reverse |
ggataacaatttcacacagg aggcaaggaaggcacaggaggcaat |
|
| BAC F15K9 |
Tail-At Forward |
cacgacgttgtaaaacgac gctgattatgggatgatgatatt |
| Tail-At Reverse |
ggataacaatttcacacagg aaaaagcagcgagttataggtttac |
|
|
Targets: corresponding GenBank accession numbers are Human coagulation factor V (Factor V, Z99573), HIV-1 Reverse Transcriptase (Reverse Trans, AF033819), Pisum sativum Retinoblastoma-related gene RBR (AB012024) and Arabidopsis thaliana BAC F15K9 sequence (AC005278). Primer sequence: in bold are indicated M13 forward and reverse universal tails; in simple text is indicated the part of the primer specific to the target sequence. | ||
Triques et al. BMC Molecular Biology 2008 9:42 doi:10.1186/1471-2199-9-42 |
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