Table 2 |
||||
|
Primers used in RT-PCR assays |
||||
|
Gene |
Sense primer |
Antisense primer |
Amplified product (bp) |
Tm (°C) |
|
|
||||
|
Ehmre11 |
5'-CGAGAAGAAGAGCAGCTCAA |
5'-CTTTCCTTTTTCTTCAGCCA |
391 |
49.5 |
|
Ehrad50 |
5'-CAGCCCAAGACATTCAAACA |
5'-CTGCATAATTGTTGTGCCAA |
277 |
49.5 |
|
Ehnbs1 |
5'-CACCTCCCACACCACAGTAT |
5'-CTCCACCAATGAATGACCAT |
549 |
49.0 |
|
Ehrad51 |
5'-ATTGCTTTTACACCAAAG |
5'-TTCTTCTGAATTTAATCC |
400 |
49.5 |
|
Ehrad51C |
5'-CCACATGACATTGTGAGTCT |
5'-GAATTATCCGATGAAGTGCT |
155 |
45.0 |
|
Ehrad52 |
5'-ATGACTGAAATAGATACCTC |
5'-AATTTGATTGTTTTAAGAAT |
730 |
37.5 |
|
Ehrad54 |
5'-GTCATGCCATTGACCAATTA |
5'-TCACACTCTTCCTCAGTTGG |
499 |
47.5 |
|
Ehrad54b |
5'-GGGCAAAAAATTCACCTAAA |
5'-GTCGTGATCCTCCAAGTGCT |
876 |
50.0 |
|
Ehrad52/22 |
5'-ATGTCTCATGAAATAAAACCAC |
5'-TCATTTCTTACGTCTAACTATTACT |
516 |
44.5 |
|
25S rRNA |
5'-TATCAAATCAAAGGACCCGCT |
5'-AAAAGA AAAACTAAGCGGTAA |
631 |
51.0 |
|
actin |
5'-AGCTGTTCTTTCATTATATGC |
5'-TTCTCTTTCAGCAGTAGTGGT |
220 |
48.0 |
|
|
||||
|
López-Casamichana et al. BMC Molecular Biology 2008 9:35 doi:10.1186/1471-2199-9-35 |
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