Table 2

Cross reaction of the miR-Q let-7 assays at different target concentrations. The percentage of cross reaction values was calculated between assay-specific and unspecific miRNA targets, while concentration of assay-specific molecules represented the full value.


let-7a miR-Q
let-7b miR-Q
let-7c miR-Q

2 nM let-7a
100
0.31
0.01
2 nM let-7b
0.00
100
0.23
2 nM let-7c
0.37
0.29
100
200 pM let-7a
100
0.31
0.01
200 pM let-7b
0.00
100
0.13
200 pM let-7c
0.24
0.24
100
20 pM let-7a
100
0.30
0.01
20 pM let-7b
0.00
100
0.10
20 pM let-7c
0.24
0.19
100
2 pM let-7a
100
0.00
0.00
2 pM let-7b
0.00
100
0.00
2 pM let-7c
0.00
0.16
100
200 fM let-7a
100
0.00
0.00
200 fM let-7b
0.00
100
0.00
200 fM let-7c
0.00
0.00
100
20 fM let-7a
100
0.00
0.00
20 fM let-7b
0.00
100
0.00
20 fM let-7c
0.00
0.00
100
2 fM let-7a
100
0.00
0.00
2 fM let-7b
0.00
100
0.00
2 fM let-7c
0.00
0.00
100

Sharbati-Tehrani et al. BMC Molecular Biology 2008 9:34   doi:10.1186/1471-2199-9-34