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Oligonucleotides used in this study. The assay-specific annealing temperatures are given in the bottom right column. Differing bases among members of the let-7 family are indicated in red. Binding sequences for universal primers are underlined and the miRNA specific sequences are indicated in bold. |
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| Synthetic miRNA molecules and DNA-oligonucleotides for RT and qPCR |
Sequence (5' to 3') |
Tanopt of miR-Q assays [°C] |
|
|
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| let-7a |
uga ggu agu agg uug uau agu u |
|
| let-7b |
uga ggu agu agg uug ugu ggu u |
|
| let-7c |
uga ggu agu agg uug uau ggu u |
|
| miR-16 |
uag cag cac gua aau auu ggc g |
|
| miR-21 |
uag cuu auc aga cug aug uug a |
|
| miR-23b |
auc aca uug cca ggg auu acc |
|
| miR-27a |
uuc aca gug gcu aag uuc cgc |
|
| miR-141 |
uaa cac ugu cug gua aag aug g |
|
| miR-145 |
guc cag uuu ucc cag gaa ucc cuu |
|
| miR-200c |
uaa uac ugc cgg gua aug aug g |
|
| RT6-let-7a |
tgt cag gca acc gta ttc acc gtg agt ggt aac tat |
|
| RT6-let-7b |
tgt cag gca acc gta ttc acc gtg agt ggt aac cac |
|
| RT6-let-7c |
tgt cag gca acc gta ttc acc gtg agt ggt aac cat |
|
| RT6- miR-16 |
tgt cag gca acc gta ttc acc gtg agt ggt cgc caa |
|
| RT6- miR-21 |
tgt cag gca acc gta ttc acc gtg agt ggt tca aca |
|
| RT6- miR-23b |
tgt cag gca acc gta ttc acc gtg agt gct ggt aat |
|
| RT6- miR-27a |
tgt cag gca acc gta ttc acc gtg agt ggt gcg gaa |
|
| RT6- miR-141 |
tgt cag gca acc gta ttc acc gtg agt ggt cca tct |
|
| RT6- miR-145 |
tgt cag gca acc gta ttc acc gtg agt ggt aag gga |
|
| RT6- miR-200c |
tgt cag gca acc gta ttc acc gtg agt ggt cca tca |
|
| short-let-7a-rev |
cgt cag atg tcc gag tag agg ggg aac ggc gtg agg tag tag gtt gta ta |
56 |
| short-let-7b-rev |
cgt cag atg tcc gag tag agg ggg aac ggc gtg agg tag tag gtt gtg tg |
56 |
| short-let-7c-rev |
cgt cag atg tcc gag tag agg ggg aac ggc gtg agg tag tag gtt gta tg |
56 |
| short-miR-16-rev |
cgt cag atg tcc gag tag agg ggg aac ggc gta gca gca cgt aaa ta |
59 |
| short-miR-21-rev |
cgt cag atg tcc gag tag agg ggg aac ggc gta gct tat cag act ga |
59 |
| short-miR-23b-rev |
cgt cag atg tcc gag tag agg ggg aac ggc gat cac att gcc agg g |
58 |
| short-miR-27a-rev |
cgt cag atg tcc gag tag agg ggg aac ggc gtt cac agt ggc taa g |
57 |
| short-miR-141-rev |
cgt cag atg tcc gag tag agg ggg aac ggc gta aca ctg tct ggt aaa g |
56 |
| short-miR-145-rev |
cgt cag atg tcc gag tag agg ggg aac ggc ggt cca gtt ttc cca gga a |
60 |
| short-miR-200c-rev |
cgt cag atg tcc gag tag agg ggg aac ggc gta ata ctg ccg ggt aa |
59 |
| 5S rRNA fw |
gcc cga tct cgt ctg atc t |
60 |
| 5S rRNA rev |
agc cta cag cac ccg gta tt |
|
| MP-fw |
tgt cag gca acc gta ttc acc |
|
| MP-rev |
cgt cag atg tcc gag tag agg |
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Sharbati-Tehrani et al. BMC Molecular Biology 2008 9:34 doi:10.1186/1471-2199-9-34 |
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