Characterization of human mesenchymal stem cell secretome at early steps of adipocyte and osteoblast differentiation

Chiara Chiellini¹ , Olivia Cochet¹ , Luc Negroni² , Michel Samson³ , Marjorie Poggi⁴ , Gérard Ailhaud¹ , Marie-Christine Alessi³ , Christian Dani¹ and Ez-Zoubir Amri¹

¹ISBDC, Université de Nice Sophia-Antipolis, CNRS ; 28 avenue de Valrose, 06100 Nice, France

²IFR 50, Faculté de Médecine, Plate-Forme Protéomique, Avenue de Valombrose, 06107 Nice, Cedex 02, France

³INSERM, Unité 638, Faculté de Médecine, Université de Nice Sophia Antipolis, Avenue de Valombrose, 06107 Nice, Cedex 02, France

⁴INSERM UMR 626; Faculté de Médecine Timone, 27 Boulevard Jean Moulin, 13385 Marseille, Cedex 5, France

author email corresponding author email

BMC Molecular Biology 2008, 9:26doi:10.1186/1471-2199-9-26


Published:	26 February 2008

Additional files

Additional file 1:

Figure 1S: LDH activity in media. LDL activity released in the medium after 6 hours of exposition to serum-free medium (white columns) compared to LDH release by complete cell lysis (black columns) from cells at day 0, day 3 adipodipocyte or osteoblast differentiation.

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Additional file 2:

Table 1S: List of peptides identified by mass spectrometry for the 73 proteins found in hMADS cell secretome. The identified proteins are indicated in bold with their corresponding peptides listed under the protein annotation. The different values correspond to parameters produced by Bioworks 3.3 after the data filtering.

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Additional file 3:

Table 2S: List of primer sequences for gene expression analysis by qRT-PCR and RT-PCR.

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