hMMS2 serves a redundant role in human PCNA polyubiquitination

Jan Brun^*¹^,2 , Roland Chiu^*³ , Katherine Lockhart⁴ , Wei Xiao⁴ , Bradly G Wouters³ and Douglas A Gray¹^,2

¹Centre for Cancer Therapeutics, Ottawa Health Research Institute, Ottawa, ON, K1H 8L6, Canada

²Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Canada

³Department of Radiation Oncology (Maastro Lab), GROW Research Institute, University of Maastricht, Maastricht, The Netherlands

⁴Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada

author email corresponding author email* Contributed equally

BMC Molecular Biology 2008, 9:24doi:10.1186/1471-2199-9-24


Published:	19 February 2008

Additional files

Additional File 1:

siRNA targeting of MMS2 and UEV1A. (A) Hela cells and (B) 293T cells were subjected to immunoblotting with an anti-Mms2/Uev1a antibody 72 hours post transfection of siRNAs targeting both MMS2 and UEV1A.

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Additional File 2:

siRNA targeting of UBC13, UEV1A and MMS2. (A) Hela cells and (B) 293T cells were subjected to immunoblotting with an anti-Ubc13 antibody 72 hours post transfection of siRNA targeting UBC13. (C) Hela cells and (D) 293T cells were subjected to immunoblotting with an anti-Mms2/Uev1a antibody 72 hours post transfection of siRNAs targeting both MMS2 and UEV1A.

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Additional File 3:

PCNA ubiquitin laddering is not altered by the knockdown of UEV1a and MMS2. Western blot analysis using an anti-PCNA antibody was performed on (A) Hela and (B) 293T protein lysates from the same samples used in the immunoprecipitations for Figure 5.

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