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Open AccessResearch article

Specificity of DNA-binding by the FAX-1 and NHR-67 nuclear receptors of Caenorhabditis elegans is partially mediated via a subclass-specific P-box residue

Stephen D DeMeo1,2* email, Rebecca M Lombel1,3* email, Melissa Cronin1 email, Eric L Smith1,4 email, Danielle R Snowflack1,5 email, Kristy Reinert1,6 email, Sheila Clever1* email and Bruce Wightman1 email

Biology Department, Muhlenberg College, Allentown, PA 18104, USA

Current address : Philadelphia College of Osteopathic Medicine, Philadelphia, PA 19131, USA

Current address : Department of Pediatrics, University of Michigan Health System, Ann Arbor, MI 48109, USA

Current address : Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA

Current address : Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA

Current address : School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA

author email corresponding author email* Contributed equally

BMC Molecular Biology 2008, 9:2doi:10.1186/1471-2199-9-2

Published: 7 January 2008

Additional files

Additional File 1:

Paired oligonucleotide sequences used for generating binding sites for EMSA and one-hybrid experiments. Complete oligonucleotide sequences evaluated in this study.

Format: PDF Size: 11KB Download file

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Additional File 2:

Supplementary methods. Details of plasmid construction and experimental conditions for EMSA.

Format: PDF Size: 24KB Download file

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