Research article
Ror2 modulates the canonical Wnt signaling in lung epithelial cells through cooperation with Fzd2
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* Corresponding author: Changgong Li changgon@usc.edu
1 Department of Pediatrics, Division of Neonatology, University of Southern California Keck School of Medicine, Los Angeles, CA 90033, USA
2 Department of Physiology and Cell Biology, Faculty of Medical Sciences, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan
BMC Molecular Biology 2008, 9:11 doi:10.1186/1471-2199-9-11
Published: 23 January 2008Additional files
Additional file 1:
Interaction of Ror2 with CRD domains of Fzd2 and Fzd7. 293T cells were transfected with expression constructs for Ror2-HA and Fzd2CRD-Flag or Fzd7CRD-Flag. Protein extracts were immunoprecipitated with anti-Flag antibodies (aFlag). Proteins associated with Protein A-sepharose were than determined by western blot with anti-HA antibody (aHA, for Ror2-HA).
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Additional file 2:
Dose response of STF to Wnt3aCM or recombinant Wnt3a protein in H441 cells. H441 cells were transfected with Fzd2 expression construct. Numbers indicate dilution range of Wnt3a CM (left) or protein concentration (ng/ml) of recombinant Wnt3a protein (right).
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