Additional file 3.

Characterization of BMAL1 antibody. (A) Western blotting. Two bands at around 70–80 kDa that correspond to BMAL1 were observed in mouse liver lysates at 4-hr intervals. CT, circadian time; arrows, BMAL1. (B) Immunoprecipitated and blotted with BMAL1 antibody. WT, liver lysate from wild-type mouse; KO, liver lysate from BMAL1-deficeient mouse. CIP, calf intestine alkaline phosphatase; arrows, BMAL1; *, a phosphorylated band.

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Nakahata et al. BMC Molecular Biology 2008 9:1   doi:10.1186/1471-2199-9-1