Additional File 2.

Cell cycle profile at 8 hours in the presence of a 2 mM thymidine post-treatment. Cells were cultured for 24 hours in the presence or absence of a 1 mM thymidine pre-treatment, electroporated with 1 μM of the oligonucleotide and then recovered in 2 mM thymidine. After an 8 hour recovery period, the cells were trypsinized and fixed to be processed for cell cycle analysis, as outlined in the methods section. When cells were not pre-treated with thymidine, the post-treatment did not alter the cell cycle profile (G1: 59%; S: 27%; G2/M: 14%). However, when the post-treatment followed the 1 mM thymidine pre-treatment, a considerable percent of cells remained in G1 or early S (G1: 62%; S: 36%; G2: 2%).

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Engstrom et al. BMC Molecular Biology 2007 8:9   doi:10.1186/1471-2199-8-9