Virtual PCR. A) Plasmid from Figure 1A, with the output options set on "Primers Analysis" showing two primers binding sites in an orientation favorable for a PCR. B) PCR window, selecting Plasmid X as the template, and the two primers. The resulting fragment can be added to the project to be further analyzed and manipulated.
Angers-Loustau et al. BMC Molecular Biology 2007 8:77 doi:10.1186/1471-2199-8-77