Table 1

Sequence of HIV-1 TAR, TAR mutants, oligonucleotides used to generate RPA probes, and synthetic siRNA

Name

Sequence

TAR-WT

GGUCUCUCUGGUUAGACCAGAUCUGAGCCUGGGAGCUCUCUGGCUAACUAGGGAACC

TAR-A

GCACAGGGAUCAAUCAGGUCUUCUCUCGCUGGGACGAGAGACCGAUUGGUCUCUUGC

TAR-B

GGUCUCUCUGGUUAGACCAGA___GAGCCUGGGAGCUCUCUGGCUAACUAGGGAACC

TAR-C

GGUCUCUCUGGUUAGACCAGAUCUGAGCCU___AGCUCUCUGGCUAACUAGGGAACC

TAR-D

GG______________CCAGAUCUGAGCCUGGGAGCUCUCUGG____________CC

TAR-E

GGUCUCUCUGGUUAGACCAGAUCUGAGCCUGGGAGCUCUCUGGCUA___________

T7 Primer

TAATACGACTCACTATAGGGAGA

TAR 5' Template

GGTCTCTCTGGTTAGACCAGATCTGATTTTTTCTCCCTATAGTGAGTCGTATTA

TAR 3' Template

AGCTCTCTGGCTAACTAGGGAACCCACTTTTTTCTCCCTATAGTGAGTCGTATTA

siGL2

5' CGUACGCGGAAUACUUCGAUU 3'

3' UUGGAUGCGCCUUAUGAAGCU 5'

siGFP

5' GCGACGUAAACGGCCACAAGUUCUC 3'

3' GCCGCUGCAUUUGCCGGUGUUCAAG 5'

siTAR1

5' UGGGUCUCUCUGGUUAGACCAGUU 3'

3' UUACCCAGAGAGACCAAUCUGGUC-P 5'

siTAR2

5' CUCUCUGGCUAACUAGGGAACCUU 3'

3' UUGAGAGACCGAUUGAUCCCUUGG-P 5'

LTR forward

CGAGCTTGCTACAAGGGACT

LTR reverse

GAGATTTTCCACACTGACTAAAAGG

Luc forward

TGAACTTCCCGCCGCCGTTGT

Luc reverse

TTACAATTTGAACTTTCCGCC

DicerRT F

TTTGTGCAGTTTCAGCTTGA

DicerRT R

CCATGGCCTTTGGAACTTC

Klase et al. BMC Molecular Biology 2007 8:63   doi:10.1186/1471-2199-8-63