Nucleotides that discriminate between binding by DMRT1 and DMRT5. (A) Comparison of sites selected by DMRT1 and DMRT5. WebLogo representation of frequency of occurrence of each base at each position in oligonucleotides selected by DMRT1 and DMRT5. Boxed residues are those selected by DMRT5 and not by DMRT1. (B) Competitive gel mobility shift assay results. Ability of unlabeled DNAs with the indicated changes to compete for binding with a labeled DMRT1 site DNA is plotted for each protein. Competition by an unaltered ("SELF") unlabelled DNA is set at 1.0 for each protein.
Murphy et al. BMC Molecular Biology 2007 8:58 doi:10.1186/1471-2199-8-58