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Primers used in the PCR experiments |
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| Fish |
Primer |
Sequence primer (5'-3') |
|
|
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| D. rerio |
DAG1_s |
CCAGCCTTTCATCTGTGGCAA |
| D. rerio |
DAG1_as |
CTTCGCACCCTTTTGGGCAC |
| D. rerio |
ACT_s |
TCTTGACCCTGAAGTACCCCATT |
| D. rerio |
ACT_as |
TCCTTGATGTCGCGCACAAT |
| D. labrax |
FISH_ext_s |
GGGCTTCAGCACATGAAGAT |
| D. labrax |
FISH_ext_as |
CTGTAGGG(A/G)GTCATGTTCTT |
| D. labrax |
ACT s |
TCCTGACCCTGAAGTACCCCA |
| D. labrax |
ACT as |
TTGATGTCACGCACGATTTCC |
| T. nigroviridis |
DAG1a s |
CAGACGTTCCTGTGTGAGGGG |
| T. nigroviridis |
DAG1a as |
GCTTCGGAAGGTGCTGCTTC |
| T. nigroviridis |
DAG1b s |
AGCTCAGCCTCTCACCTGTAGC |
| T. nigroviridis |
DAG1b as |
GACTCGTTTCACTCCATGGACC |
| T. nigroviridis |
ACT s |
CACCCTGAAGTATCCCATCGAA |
| T. nigroviridis |
ACT as |
GTCTCTGACGATCTCTCGCTCAG |
|
The primers used to amplify the gene sequences from D. rerio and T. nigroviridis were designed using the sequences available in the database. The degenerate primers FISH_ext_s and FISH_ext_as, which allowed the fishing of the newly identified D. labrax sequence, were chosen exploiting two regions displaying very high homology within the aligned DG sequences from D. rerio and T. rubripes (see also Fig. 1). | ||
Pavoni et al. BMC Molecular Biology 2007 8:34 doi:10.1186/1471-2199-8-34 |
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