Multiple alignment of dystroglycan amino acid sequences obtained using the ClustalW software. The DG protein sequences from T. rubripes, T. nigroviridis, O. latipes and G. aculeatus are the conceptual translations of genomic available DNA sequences. Identical residues are highlighted in yellow. The cyan highlighting identifies the first intron insertion site and the red highlighting identifies the insertion site of the mini-intron. It should be noted that due to some possible sequencing mistakes, the 3' end of T. rubripes DAG1a, and therfore the corresponding C-terminal amino acid sequence, is not fully available in the Ensembl databank. The α/β cleavage site is also highlighted (black) while the green highlighting identifies the β-DG binding epitope and the cyan one the α-DG binding epitope [39,40]. The regions chosen for designing the two primers (FISH_ext_s and FISH_ext_as) used for the DG-homologous cloning experiment in D. labrax are indicated by red arrows.
Pavoni et al. BMC Molecular Biology 2007 8:34 doi:10.1186/1471-2199-8-34