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Open Access Research article

Deletion of the cruciform binding domain in CBP/14-3-3 displays reduced origin binding and initiation of DNA replication in budding yeast

Wafaa Yahyaoui1, Mario Callejo1, Gerald B Price12 and Maria Zannis-Hadjopoulos1*

Author Affiliations

1 McGill Cancer Centre, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada

2 In memoriam

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BMC Molecular Biology 2007, 8:27  doi:10.1186/1471-2199-8-27

Published: 12 April 2007

Abstract

Background

Initiation of eukaryotic DNA replication involves many protein-protein and protein-DNA interactions. We have previously shown that 14-3-3 proteins bind cruciform DNA and associate with mammalian and yeast replication origins in a cell cycle dependent manner.

Results

By expressing the human 14-3-3ε, as the sole member of 14-3-3 proteins family in Saccharomyces cerevisiae, we show that 14-3-3ε complements the S. cerevisiae Bmh1/Bmh2 double knockout, conserves its cruciform binding activity, and associates in vivo with the yeast replication origins ARS307. Deletion of the α5-helix, the potential cruciform binding domain of 14-3-3, decreased the cruciform binding activity of the protein as well as its association with the yeast replication origins ARS307 and ARS1. Furthermore, the mutant cells had a reduced ability to stably maintain plasmids bearing one or multiple origins.

Conclusion

14-3-3, a cruciform DNA binding protein, associates with yeast origins of replication and functions as an initiator of DNA replication, presumably through binding to cruciform DNA forming at yeast replicators.