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Relative mRNA levels of C-28/I2 chondrocytes under the influence of IL-1β and diacerein. |
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| Experimental condition |
mRNA relative to untreated control group (mean ± SD) |
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| ACTB |
GAPDH |
B2M |
HPRT1 |
SDHA |
YWHAZ |
|
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|
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| IL-1β |
1.5 ± 0.63 |
1.2 ± 0.57 |
1.10 ± 0.08 |
1.23 ± 0.46 |
1.02 ± 0.66 |
0.89 ± 0.45 |
| Simultaneous incubation with diacerein [c]1 |
1.03 ± 0.39 |
1.11 ± 0.40 |
1.36 ± 0.29 |
1.26 ± 0.40 |
1.13 ± 0.34 |
1.14 ± 0.43 |
| Simultaneous incubation with diacerein [c]2 |
0.71 ± 0.18 |
1.02 ± 0.21 |
1.15 ± 0.20 |
1.07 ± 0.25 |
0.88 ± 0.17 |
0.69 ± 0.17 |
| Pre-incubation with diacerein [c]1 |
1.21 ± 0.21 |
1.12 ± 0.33 |
1.23 ± 0.57 |
1.29 ± 0.40 |
1.35 ± 0.39 |
1.11 ± 0.31 |
| Pre-incubation with diacerein [c]2 |
0.56 ± 0.07 |
0.79 ± 0.11 |
0.96 ± 0.12 |
1.00 ± 0.13 |
0.98 ± 0.19 |
0.7 ± 0.15 |
| Diacerein only |
0.43 ± 0.06* |
0.63 ± 0.10 |
0.73 ± 0.09 |
0.87 ± 0.15 |
0.88 ± 0.15 |
0.59 ± 0.07 |
|
Boldfaced values p < 0.05 * p < 0.05 and change > 2-fold mRNA levels under the treatment with diacerein in different experimental conditions are presented relative to the untreated control group calculated by the equation E-ΔCt (Mean ± SD, n = 9; value of 1 represents average level of mRNA expression in untreated control group). [c]1, low concentration of diacerein (5 μM); [c]2, high concentration of diacerein (50 μM). | ||||||
Toegel et al. BMC Molecular Biology 2007 8:13 doi:10.1186/1471-2199-8-13 |
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