Table 3

Overall comparison of the four RNA extraction protocols for quantity (in pg/μl) and quality (RIN) for ten year old FFPE samples.

Ten year old samples:

Protocol 1

Protocol 2

Protocol 3

Protocol 4


FFPE tissue input

80 μm

10 μm

20 μm

50 μm

Successful extractions

36/42 (86%)

26/42 (62%)

38/42 (90%)

30/42 (71%)

RNA (pg/μl)

Minimum value

975

1,650

1,314

9864

Maximum value

73,861

165,588

137,594

314,693

Mean

15,964

51,985

29,661

107,042

Median

8,750

31,104

19,458

86,682

RIN

Minimum value

1.0

1.5

1.0

1.8

Maximum value

2.5

5.2

4.4

6.1

Mean

1.8

2.5

2.3

2.4

Median

2.0

2.3

2.2

2.2


Ribeiro-Silva et al. BMC Molecular Biology 2007 8:118   doi:10.1186/1471-2199-8-118

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