|
Overall comparison of the four RNA extraction protocols for quantity (in pg/μl) and quality (RIN) for ten year old FFPE samples. |
|||||
| Ten year old samples: |
Protocol 1 |
Protocol 2 |
Protocol 3 |
Protocol 4 |
|
|
|
|||||
| FFPE tissue input |
80 μm |
10 μm |
20 μm |
50 μm |
|
| Successful extractions |
36/42 (86%) |
26/42 (62%) |
38/42 (90%) |
30/42 (71%) |
|
| RNA (pg/μl) |
Minimum value |
975 |
1,650 |
1,314 |
9864 |
| Maximum value |
73,861 |
165,588 |
137,594 |
314,693 |
|
| Mean |
15,964 |
51,985 |
29,661 |
107,042 |
|
| Median |
8,750 |
31,104 |
19,458 |
86,682 |
|
| RIN |
Minimum value |
1.0 |
1.5 |
1.0 |
1.8 |
| Maximum value |
2.5 |
5.2 |
4.4 |
6.1 |
|
| Mean |
1.8 |
2.5 |
2.3 |
2.4 |
|
| Median |
2.0 |
2.3 |
2.2 |
2.2 |
|
Ribeiro-Silva et al. BMC Molecular Biology 2007 8:118 doi:10.1186/1471-2199-8-118 |
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