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Research article

The venom gland transcriptome of the Desert Massasauga Rattlesnake (Sistrurus catenatus edwardsii): towards an understanding of venom composition among advanced snakes (Superfamily Colubroidea)

Susanta Pahari¹ , Stephen P Mackessy² and R Manjunatha Kini³

¹Center for Post Graduate Studies, Sri Bhagawan Mahaveer Jain College, 18/3, 9th Main, Jayanagar 3rd Block, Bangalore, India

²School of Biological Sciences, University of Northern Colorado, Greeley, CO 80639-0017, USA

³Protein Science Laboratory, Department of Biological Sciences, National University of Singapore, Singapore 117543 and Deparment of Biochemistry, Virginia Commonwealth University, Medical college of Virginia, Richmond, VA 23298-0614, USA

author email corresponding author email

BMC Molecular Biology 2007, 8:115doi:10.1186/1471-2199-8-115


Published:	20 December 2007

Additional files

Additional file 1:

It is a table showing the distribution of snake venom toxin families among Superfamily Colubroidae.

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Additional file 2:

It is a table showing the clusters of ESTs, number of clones in each cluster and their putative identity.

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Additional file 3:

It is a table showing the clusters of ESTs encoding cellular proteins.

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Additional file 4:

ClustalW alignment between 12S ribosomal RNA sequence DQ464268 (from this study) and AF057227 (used for taxonomic identification of S. c. edwardsii). Polyadenylation signal sequence is underlined.

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Additional file 5:

ClustalW alignment of PIII metalloproteinases (only proteinase domain is shown). Cysteine residues which are conserved are marked in grey and variable in black. Accession numbers of the used sequences are as follows: VAP1 [GenBank: BAB18307], HV1 [GenBank: BAB60682], Halysase [GenBank: 27465044], VLAIP-A [GenBank: 61104775], VLAIP-B [GenBank: 61104777], Kaouthiagin [Swiss-Prot: P82942], Berythractivase [Swiss-Prot: Q8UVG0], Ecarin [Swiss-Prot: Q90495], Jararhagin [Swiss-Prot: P30431], Bothropasin [Swiss-Prot: O93523], Acurhagin [Swiss-Prot: Q6Q274], Catrocollastatin [Swiss-Prot: Q90282], Atrolysin [Swiss-Prot: Q92043], Stejnihagin-A [Swiss-Prot: Q3HTN1], Stejnihagin-B [Swiss-Prot: Q3HTN2], HR1A [Swiss-Prot: Q8JIR2], HR1B [Swiss-Prot: P20164], HF3 [GenBank: 31742525]. P, signal peptide domain; PRO, pro-domain; S, spacer; DISIN, disintegrin domain; CRD, cysteine-rich domain

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Additional file 6:

Bayesian tree generated from 39 aligned 3FTx sequences as described in Materials and Methods. Numbers on branches indicate percentage of posterior clade probability. 3FTx sequences from S. c. edwardsii and L. muta libraries are marked with a filled circle and triangle respectively.

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