Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis
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* Corresponding author: Štefan Čikoš cikos@saske.sk
BMC Molecular Biology 2007, 8:113 doi:10.1186/1471-2199-8-113
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Robert G Rutledge, Don Stewart BMC Molecular Biology 2008, 9:96 (30 October 2008) Measurement of template quantity in real-time qPCR using standard curves is prone to error, and the novel linear regression of efficiency method that calculates the amplification efficiency at the onset of each reaction is more accurate.
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Robert G Rutledge, Don Stewart BMC Biotechnology 2008, 8:47 (8 May 2008) The LRE (linear regression of efficiency) algorithm for estimating PCR efficiency is a kinetic-based sigmoidal approach that, when coupled with optical calibration, provides data comparison without using standard curves, allowing high-capacity absolute PCR quantification.
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