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Open Access Highly Accessed Research article

Translation initiation region sequence preferences in Escherichia coli

Vladimir Vimberg1, Age Tats2, Maido Remm2 and Tanel Tenson1*

Author Affiliations

1 Institute of Technology, University of Tartu, Nooruse 1, Tartu 50411, Estonia

2 Department of Bioinformatics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, Tartu 51010, Estonia

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BMC Molecular Biology 2007, 8:100  doi:10.1186/1471-2199-8-100

Published: 31 October 2007

Additional files

Additional file 1:

Sequences of the TIRs used in the study. Sequences of the TIRs are provided.

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Additional file 2:

Reverse transcription real-time PCR to determine mRNA levels. The file contains information about mRNA levels.

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Additional file 3:

The effect of the TIR on GFP synthesis at 37°C. The experimental data used for Figure 2 are provided in fluorescence units. In addition, the data for araBAD promoter are shown.

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Additional file 4:

The effect of the TIR on GFP synthesis at 20°C. The data from measurements done at 20°C are provided for all enhancer contexts.

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Additional file 5:

Plasmids used for GFP expression. Sequences of the plasmids are provided.

Format: DOC Size: 34KB Download file

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Open Data