Conversion of any Gateway destination vector to a MultiSite Gateway destination vector. (A) LR recombination of the pDONR-R4-R3 vector with a linear Gateway destination vector. We tested this reaction using pDEST32 (Invitrogen) and pDEST22 (Invitrogen) destination vectors. (B) LR Plus recombination of three entry clones bearing the DNA fragments "A", "B" and "C" with a converted MultiSite Gateway destination vector. We tested this reaction by recombining three entry clones carrying a 3434 bp, 3116 bp and 561 bp DNA fragment with pDEST32-R4-R3 and pDEST22-R4-R3. (C) DNA and amino acid sequence of the external borders of the expression clone.
Magnani et al. BMC Molecular Biology 2006 7:46 doi:10.1186/1471-2199-7-46