Table 1 |
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|
Summary of results for mTARC putative STAT6 sites |
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|
Putative site |
Position within mTARC promoter |
Sequence |
Significance inferred from 5 'deletion series |
Binds STAT6 in EMSA |
Trimer construct induced by IL-4 in transfections |
Reduction in IL-4 induction of mTARC -1173 when site mutated (approx.) |
|
|
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|
T1 |
-140 to -148 |
TTCTTTGAA* |
+ |
+ |
- |
85% |
|
T2 |
-194 to -203 |
TTCTCTGGAA |
+ |
+ |
++ |
100% |
|
T3 |
-473 to -482 |
ATCCCTGTAA |
- |
- |
ND |
ND |
|
T4 |
-484 to -493 |
TTCTACTGAA |
- |
+ |
+ |
40% |
|
T5 |
-513 to -522 |
TTCCCCAAAA |
+ |
- |
ND |
60% |
|
T6 |
-531 to-540 |
TTCAGCTGAA |
+ |
- |
- |
60% |
|
T7 |
-657 to -666 |
TTTCTGGGAG |
- |
- |
ND |
ND |
|
T8 |
-763 to -111 |
TTCTAGGGCA |
- |
- |
ND |
ND |
|
T9 |
-854 to -863 |
TTCTGGGCAC |
- |
- |
ND |
ND |
|
T10 |
-870 to -879 |
TTGTCCTGAA |
- |
- |
ND |
ND |
|
T11 |
-904 to -913 |
TTCTCAGTAA |
- |
+ |
++ |
15% |
|
|
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|
*residues in bold conform to the core consensus STAT6 binding site. + indicates positive result. - indicates negative result. ++ indicates highly activated by IL-4. ND indicates experiment not done. |
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|
Liddiard et al. BMC Molecular Biology 2006 7:45 doi:10.1186/1471-2199-7-45 |
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