Figure 8.

mTARC STAT6 sites on heterologous minimal promoters are potent IL-4 responders. RAW 264.7 cells were transfected as described in Figure 4A with 5 μg promoter-luciferase constructs indicated and 3 μg STAT6 expression vector. E denotes the Emr1 minimal promoter and A denotes the EIF-4A1 minimal promoter. The 5XST6 constructs consist of 394 bp murine TARC sequence from position -132 (refer to Figure 3) to -526 and contain 5 of the putative STAT6 sites. The 9XST6 constructs consist of 736 bp murine TARC sequence from position -132 (refer to Figure 3) to -868 and contain 9 of the putative STAT6 sites. F refers to the forward orientation of the promoter. Cells were treated +/- 20 ng/ml recombinant murine IL-4 (A) or IL-4 doses stated (B) for 18 hours before being harvested and assayed for luciferase and β-galactosidase activity. Data are representative of at least 2 transfections (A) or standardised results from multiple transfections (B) and results are displayed as fold induction of sample relative luciferase activity (luciferase/β-galactosidase) with cytokine treatment +/- SD.