Research article
Interleukin-4 induction of the CC chemokine TARC (CCL17) in murine macrophages is mediated by multiple STAT6 sites in the TARC gene promoter
-
* Corresponding author: David R Greaves david.greaves@path.ox.ac.uk
1 Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
2 Department of Cellular and Molecular Medicine and Department of Medicine, UCSD, La Jolla, California 92093-0651, USA
BMC Molecular Biology 2006, 7:45 doi:10.1186/1471-2199-7-45
Published: 29 November 2006Additional files
Additional File 1:
Regulation of mTARC expression by IL-4 and IFN-γ. This figure shows the expression of mTARC and IP-10 mRNAs in total RNA prepared from peritoneal macrophages treated with the indicated amounts of recombinant murine IL-4 or IFN-γ.
Format: PPT Size: 86KB Download file
This file can be viewed with: Microsoft PowerPoint Viewer
Additional File 2:
De novo protein synthesis is not required for the IL-4 induction of mTARC. This figure shows mTARC mRNA expression in C57BL/6 peritoneal thioglycollate-elicited Mθ that were pre-treated with either 0, 0.5 or 1 μg/ml cycloheximide for 2 hours. Cells were then treated +/- 20 ng/ml recombinant murine IL-4 for a further 4 or 24 hours. Total RNA was harvested and TARC cDNA copy number was measured by real- time PCR +/- SEM. Results are representative of at least 3 similar experiments.
Format: PPT Size: 19KB Download file
This file can be viewed with: Microsoft PowerPoint Viewer