Figure 4.

Analysis of Licpa null mutants. A) PCR analysis of the two ΔLicpa mutants. PCR reactions were performed using primers indicated in Figure 3A. WT: wild-type, H; heterozygote CPA/ΔLicpa, ΔC1: ΔLicpaC1 and ΔC2: ΔLicpaC2. B) Southern blot analysis of the ΔLicpaC1 (lane A) and ΔLicpaC1::CPA (lane B) re-expresser mutant DNA digested with PstI and hybridized with a 5'FR probe. C) RT-PCR analysis of wild type (WT), ΔLicpaC1 (ΔC1), ΔLicpaC1::CPA (ΔC1:CPA), ΔLicpaC2 (ΔC2) and ΔLicpaC2::CPA (ΔC2:CPA) parasites. Nested PCR were carried out in presence (+) or in absence (-) of cDNA using the primer pairs OL136/SL primer. Lower panel is a control with the LinJ36.2050 gene.