Figure 1.

Alignment of CPA amino acid sequences. Alignment of deduced amino acid sequence of LiCPA (CAD12932) from L. infantum JPCM5, with deduced amino acid sequences obtained from GenBank. These included L. major (LmjCPA, CAC18865.1), L. mexicana (lmxCPA, CAA44094), L. donovani (LdCPA, AAK27384.1), L. chagasi (LdcCPA, AAC38833) and L. braziliensis (LbCPA, LbrM19.1180). The dots represent amino acid identity. The arrows show the predicted cleavage sites of pro/mature domains and mature/C-terminal extension, respectively. Amino acid residues important for the catalysis are indicated as follows : active site cysteine C¹⁵³ (◇), the predicted catalytic dyads H²⁸⁹ and N³⁰⁹ (●) and the predicted glutamine of the oxyanion hole Q¹⁴⁷ (▼). The potential glycosylation sites are shown in boxes.