Table 1 |
||||
|
Transformation efficiency of the E. coli lysogens carrying stx2-phages |
||||
|
pBC-SK+ |
pKD46 |
|||
|
Vector + |
stx+ |
Vector + |
stx+ |
|
|
|
||||
|
DH5α (VTB55) |
99.0a |
100.0 |
100 |
6 |
|
DH5α (312) |
98.5 |
96.5 |
100 |
1 |
|
DH5α (534) |
100.0 |
99.5 |
100 |
80 |
|
DH5α (549) |
97.5 |
100.0 |
100 |
5 |
|
DH5α (557) |
100.0 |
100.0 |
100 |
3 |
|
C600(A9) |
99.2 |
100.0 |
100 |
100 |
|
C600(933W) |
100.0 |
100.0 |
100 |
100 |
|
|
||||
|
aPercentage of colonies positive for the presence of each vector performed in parallel in two plates, relative to the total number of colonies grown in plates with the respective antibiotic. Results were obtained by colony hybridization of the colonies present in a plate. |
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|
Serra-Moreno et al. BMC Molecular Biology 2006 7:31 doi:10.1186/1471-2199-7-31 |
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