Table 2

Female gametophytic defects in the Atmnd1 mutant.

Ovule Stages

1n

2n

4n

8n

MES

Degen

m

+

m

+

m

+

m

+

m

+

m

+

3-1

31

100

ND

ND

ND

ND

ND

ND

ND

ND

69

ND

3-2

25

60

9

40

ND

ND

ND

ND

ND

ND

66

ND

3-4

39

2

4

40

7

52

ND

5

ND

ND

50

0.8

3-6

32

ND

6

2

0.4

7

0.2

7

0.6

83

60

1.2

Stages of female gametophyte development relative to ovule stages in the Atmnd1 mutant and wild type. A total of 759 and 637 ovules were scored for the Atmnd1 mutant and wild-type respectively. Megagametogenesis defects are evident from stage 3-1 in case of the mutant, which leads to embryo sac arrest and eventually its degeneration. Ovule stages are according to [54]: 3-1, 3-2, and 3-4 are postmeiotic stages of the sporophyte with respect to elongation of the integuments where the wild type gametophyte would normally reach the 1n, 2n, and 4n stages; 3-6 corresponds to a mature ovule prior to fertilization. MES, Mature embryo sac; Degen, Degenerated embryo sac; M, mutant; +, wild type; ND, Not detected.

Panoli et al. BMC Molecular Biology 2006 7:24   doi:10.1186/1471-2199-7-24