Table 1 |
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Oligonucleotides used as primers in RT-PCR analysis |
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Target mRNA |
Primer Sequence |
Amplicon Size (bp) |
|
|
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|
TPS1 |
Forward CGCTAAGGCGCAACTGACCTCGTCT Reverse CGACGAGAATGCGTGGTTGGCATAC |
379 |
|
TPS2 |
Forward CCCCCCAAACTATCAGATTGGAACAAC Reverse ACCCAGCTGCAGCTATTCCATCGGC |
612 |
|
PDA1 |
Forward GGTCAGGAGGCCATTGCTGT Reverse GACCAGCAATTGGATCGTTCTTGG |
673 |
|
CNA1 |
Forward CGAAAGACTTGAATTCTTCACGCATC Reverse GAATGATCTGCAGCAAGCATCG |
629 |
|
CNA2 |
Forward CCTTATATCTGTTCCCGCCC Reverse GAGGAACCATGGTTTTGGAG |
551 |
|
ACT1 |
Forward CCTACGTTGGTGATGAAGCT Reverse GTCAGTCAAATCTCTACCGG |
407 |
|
|
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CNA1, CNA2, PDA1 and ACT1 primer sequences were obtained from Del Aguila et al, 2003 and TPS1 and TPS2, from Souza, 2001. |
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Del Aguila et al. BMC Molecular Biology 2005 6:9 doi:10.1186/1471-2199-6-9 |
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