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SiteFind: A software tool for introducing a restriction site as a marker for successful site-directed mutagenesis

Paul M Evans and Chunming Liu*

BMC Molecular Biology 2005, 6:22  doi:10.1186/1471-2199-6-22

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Methodology - explanation reqd...

Natarajan Ganesan   (2006-04-21 15:56)  Georgetown University email

How is the match found for a given sequence? Do you connect the program to a database of restriction enzymes? I couldnt find the description in Methods.

Whatever be the approach to find a match, I feel the need to connect a restriction enzyme database to compute the ideal enzyme and an ideal insert.

As I see, one possible approache would allow the user to select an enzyme and then look for a match that retains the amino-acid sequence (using degeneracy).

Sincerely Yours

Natarajan Ganesan, PhD

Email: ng6@georgetown.edu

Competing interests

No competing interests

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