The C-terminal fragment of Cdc23S424::Tcs is not localized to the nucleus after cleavage by TEV protease. A. cdc23S424::Tcs strain (P1323) was grown in plus (-TEV) or minus thiamine (+TEV) media at 25°C for 24 h, then shifted to 37°C for 4 h before fixation with methanol and acetone. Panels show Cdc23-CFP fluorescence and DAPI staining superimposed on phase images. Bar = 10 μm. B. Quantitation of experiment shown in A. 100 cells were counted for each time point.
Yang et al. BMC Molecular Biology 2005 6:13 doi:10.1186/1471-2199-6-13