Figure 3.

Flow cytometry analysis of cdc23_S424::Tcs strains following induction of TEV protease. A. Scheme for experiment shown in (B) & (C). B-C. Flow cytometric analysis of G1-arrested cdc23_S424::Tcs strain (P1376), released from a G1 block in the absence (B) or presence (C) of thiamine, showing arrest of DNA replication when TEV protease is expressed. D. Flow cytometric analysis of cdc23_S424::Tcs strain (P1376) expressing TEV protease (i.e. – thiamine). The strain was grown to log phase at 25°C in – thiamine medium and shifted to 37°C at t = 0. The top panel superimposes the t = 0 (grey) and t = 3 h (black) histograms.