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Characterization of TRZ1, a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z

BMC Molecular Biology volume 6, Article number: 12 (2005) Cite this article

Abstract

Background

In humans, mutation of ELAC2 is associated with an increased risk of prostate cancer. ELAC2 has been shown to have tRNase Z activity and is associated with the γ-tubulin complex.

Results

In this work, we show that the yeast homolog of ELAC2, encoded by TRZ1 (tRNase Z 1), is involved genetically in RNA processing. The temperature sensitivity of a trz1 mutant can be rescued by multiple copies of REX2, which encodes a protein with RNA 3' processing activity, suggesting a role of Trz1p in RNA processing in vivo. Trz1p has two putative nucleotide triphosphate-binding motifs (P-loop) and a conserved histidine motif. The histidine motif and the putative nucleotide binding motif at the C-domain are important for Trz1p function because mutant proteins bearing changes to the critical residues in these motifs are unable to rescue deletion of TRZ1. The growth defect exhibited by trz1 yeast is not complemented by the heterologous ELAC2, suggesting that Trz1p may have additional functions in yeast.

Conclusion

Our results provide genetic evidence that prostate cancer susceptibility gene ELAC2 may be involved in RNA processing, especially rRNA processing and mitochondrial function.

Background

With a cumulative incidence by age 75 of 14% among Caucasian-Americans and 25% among African-Americans, prostate cancer has become the most common non-cutaneous malignancy in the US [1]. Despite its high incidence rate, there is little known about the genetics and etiology of prostate cancer. We recently identified a candidate prostate cancer susceptibility gene through linkage analysis and positional cloning [2]. This gene, ELAC2, is located on chromosome 17p and encodes a protein of 826 amino acids with tRNase Z activity (tRNA 3' endoribonuclease activity) [3]. ELAC2 belongs to an incompletely characterized family of proteins that is conserved among eukaryotes, archaea and bacteria. ELAC2 is ubiquitously expressed in all tissues with the highest expression levels found in testes. Elac2p is detected in several cancer cell lines and binds the γ-tubulin complex [4]. At present the role that sequence variants in ELAC2 may play in the genesis of prostate cancer is unclear.

TRZ1 (tRNase Z 1, YKR079c), the yeast ortholog of ELAC2, encodes an essential protein of 838 amino acids. Like ELAC2 and its other eukaryotic orthologs, TRZ1 can be divided roughly into amino- and carboxy-halves, or domains, which are structurally homologous [2]. The carboxy domain possesses tRNase Z activity; the amino domain is enzymatically inactive but may modulate the specificity of carboxy domain. The most strongly conserved sequence element is the histidine motif, located near the front of the carboxy domain. In the amino domain, the structural homolog of the histidine motif is easily recognizable; however, most of the key zinc coordinating histidines have changed to some other polar amino acid, which is entirely consistent with the observation that this domain lacks tRNase Z activity. PROSITE scans revealed that the vertebrate ELAC2s contain a sequence element near the center of their amino domain that matches the nucleotide binding P-loop motif [2]. Although not exactly matching the canonical P-loop sequence, this sequence element is well conserved in TRZ1 and its structural homolog is well conserved near the center of the carboxy domain of all gene family members. It has recently been shown that both of the two ELAC family members (tRNase ZL and tRNase ZS) from the Animals, Plants, Fungi, Bacteria, and Archaea possess tRNase Z activity [3, 5–9]. The amino acid and enzymatic conservation across ELAC family members suggests strongly that they all share some basic cellular functions. Since Saccharomyces cerevisiae is a well-characterized genetic system, we initiated a genetic analysis of the functions of the yeast member of this gene family, TRZ1, in order to better understand those of ELAC2.

RNA processing, one of the post-transcriptional controls on gene expression, plays an important role in regulating cell growth and fate. Pre-mRNA processing is a multi-step process coupled to transcription through the involvement of the unique C-terminal domain (CTD) of the large subunit of RNA polymerase II. The processing involves RNA binding proteins, snRNAs (small nuclear RNAs), endo- and exo-nucleases, and other specific factors. The function of these proteins is subject to regulation by different signaling pathways, according to the developmental stage of the cell [10].

Transfer RNAs (tRNAs) undergo extensive post-transcriptional modifications, including: 5' processing; 3' processing; nucleotide modification; and, in the case of about 20% of yeast tRNA, intron removal in the nucleus before export to the cytosol. RNase P (ribonuclease P), which consists of a RNA subunit and nine proteins, is responsible for 5' tRNA processing (reviewed in [11]). Two proteins, Trz1p [3] and Lhp1p [12], have been shown to be involved in 3' tRNA maturation in yeast; Trz1p has tRNase Z activity while Lhp1p stabilizes pre-tRNAs as an RNA chaperone [3, 12]. It is known that there are more than 55 proteins involved in tRNA splicing, modification, degradation and export.

Ribosomal RNAs (rRNAs) are transcribed as large precursors by RNA polymerases I and III in nucleoli [13]. These RNAs also undergo processing, including 5' and 3' cleavages, removal of introns, and nucleotide modifications through the action of a series of RNA/protein complexes. The mature rRNAs complex with about 80 proteins to form ribosomes. The RNase MRP (ribonuclease MRP) complex is responsible for the majority of 5' maturation steps and the exosome complex is responsible for 3' rRNA maturation. Many of the modifications and splicing events require small nucleolar RNAs (snoRNAs) and other specific protein factors. Notably, some proteins are shared between the tRNA and rRNA maturation complexes (reviewed in [14]).

In this paper we show that, although Trz1p is required for growth, yeast are largely insensitive to the absolute level of its expression. ELAC2 cannot functionally replace Trz1p, indicating that Trz1p has additional or specific functions. In the carboxy domain of the protein, the conserved histidine motif and the putative P-loop are important for Trz1p biological function because mutations of the conserved residues in these motifs render the protein inactive in yeast. Through a high copy suppressor screen, we found that TRZ1 genetically interacts with REX2, suggesting a role for TRZ1 in RNA processing and mitochondrial maintenance.

Results

Effect of altered TRZ1 expression on yeast cell growth

We generated three yeast strain types in order to study the consequences of altered TRZ1 expression in yeast. In the first (YL09-01 and YL09-02), TRZ1 is expressed from a Gal1 promoter on a low-copy plasmid in a wild type TRZ1 background. In the second (YL10-04 and YL10-05), the GAL1 promoter is inserted upstream of TRZ1 between the translational start site and its endogenous 5'UTR at the chromosomal locus (no deletion of the endogenous locus). In the third strain type (YL10-01, YL10-02 and YL10-03), endogenous TRZ1 is deleted and the gene is expressed from a GAL1 promoter on a low-copy plasmid. The latter two strain types were made to investigate the effect of up- and down- regulation of TRZ1 expression on growth. The genotypes of all strains used in this study are listed in Table 1. Also listed in Table 1 is the ρ +/ρ - status of each strain, assayed as the ability of the strain to grow on media containing only non-fermentable carbon sources. The significance of the ρ +/ρ - status, which represents the wild type (ρ+) or impaired (ρ-) (petite mutant) mitochondrial respiratory function, will become clear below.

Table 1 Strains used in this study
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As shown in Table 2, when TRZ1 is over-expressed in a wild type background (YL09-01), the yeast doubling time is 3.7 hours (average of 2 assays, 3.85 hours in one experiment and 3.55 hours in another experiment), not significantly longer than that of YPH499, the parental yeast control (3.4 hours; average of 2 assays, 3.11 hours in one experiment and 3.75 hours in another experiment) or that of wild type yeast that over-express β-galactosidase (3.0 hours, one assay only). Therefore, over-expression of TRZ1 in wild type yeast does not significantly slow yeast growth. Overexpression of FLAG-TRZ1 in YL09-02 was verified by Western blot with anti-FLAG antibody. As shown in Figure 1a, FLAG -TRZ1 is rapidly induced in YL09-02 grown on galactose-containing medium. Notably, both YL09-01 and YL09-02 are ρ+.

Table 2 doubling time (hour)
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Figure 1
figure 1

Induction of exogenous FLAG•Trz1p. A. Growth of YL09-02 was initiated in raffinose complete medium. After overnight growth at 30°C, the culture was diluted with raffinose complete medium to O.D. 0.2–2.3 at 600 nm, grown to O.D. 0.4, then induced in exponential growth phase with 2% galactose for varying periods of time before processing for Western blot. B. YPH499 and YL10-04 were grown as described above and induced with 2% galactose for 4 hours before harvesting for Western blot. Arrow indicates FLAG•Trz1p.

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We then investigated the consequence of over-expression of TRZ1 from the chromosomal locus. In YL10-04 a GAL1 promoter is inserted immediately upstream of the translational start codon of FLAG-TRZ1. As shown in Figure 1b, FLAG-TRZ1 is promptly induced after addition of galactose in the YL10-04 strain. Liquid growth assays in a variety of different media were done to determine the doubling time of strains expressing varying levels of Trz1p (Table 2). No growth was observed for YL10-04 and YL10-05 in galactose medium, respectively, compared to 3.75 hr doubling time forYPH499. However, these GAL1 promoter insertion strains also grew more slowly in non-inducing medium. The doubling time for YL10-04 and YL10-05 in glucose medium is 3 and 2 times longer than that of parental YPH499 cells (Table 2). We experienced difficulties when trying to isolate Gal1 promoter insertion strains and found that the isolated strains were petite mutants; therefore, we decided not to study these strains further.

We observed the growth characteristics of trz1 strains with TRZ1 expressed from a GAL1 promoter on a low-copy plasmid (YL10-01, YL10-02 and YL10-03). As shown in Table 2, when TRZ1 expression is repressed in glucose medium, YL10-01 grows marginally slower (doubling time = 2.95 hours) than YPH500, the isogenic wild type yeast (doubling time = 2.27 hours). The same is true for YL10-02 and YL10-03 with respect to YPH499, their isogenic wild type strain. Thus, although TRZ1 is an essential gene, even low levels of its transcript are sufficient for near normal growth. When TRZ1 expression is induced in galactose medium, however, YL10-01 and YL10-02 fail to grow, while YL10-03 grows at about the same rate as wild type yeast. This is intriguing, particularly when comparing YL10-02 and YL10-03, because the strains share the same genetic background. They differ in two ways: YL10-02 is ρ- (petite phenotype) while YL10-03 is ρ+ (table 1); and YL10-02 expresses untagged TRZ1 while YL10-03 expresses TRZ1 tagged with a FLAG epitope. The phenotypic discrepancy between YL10-02 and YL10-03 is not likely due to a lack of expression of Trz1p or lack of functional Trz1p in YL10-03, because we could detect FLAG•TRZ1p in YL10-03 (data not shown) and p414/Gal1p-FLAG•TRZ1 could functionally complement the trz1 deletion (Table 4). The difference between the strains is more likely due to their ρ status.

Table 3 growth comparison on different sugar
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Table 4 Complementation of trz1
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We studied these strains further by testing the effect of different carbon sources on their growth. Table 3 summarizes these results. When TRZ1 expression from a Gal1p-driven TRZ1 plasmid is suppressed on SC-glucose, wild type yeast (YL09-01) and trz1 yeast (YL10-03) grow at a rate similar to that of YPH499 at room temperature, 30°C and 37°C. We could not detect any significant difference in growth rate among YPH499, YL09-01 and YL10-03 on sucrose, raffinose or galactose at any temperature tested (Table 3). This result agrees with the results from liquid growth assays in that over-expression of TRZ1 in these two strains (YL09-01 and YL10-03) does not result in any discernable change in growth rate. When spotted onto glucose media and incubated at room temperature, YL10-01 and YL10-02 grow at a rate equivalent to that of YPH499. As the incubation temperature is increased, however, YL10-01 and YL10-02 grow significantly slower than YPH499. When spotted on sucrose media, these strains grow slower than YPH499 at all tested temperatures, with the most significant difference in doubling time occurring at 37°C. YL10-01 and YL10-02 barely grow on raffinose and do not grow at all on galactose. As argued above, the phenotypic discrepancies among the strains is not likely due to differences in expression of functional TRZ1p. Instead, the different phenotypes resulting from galactose-induced expression of Trz1p is more likely attributable to the ρ+/ρ- status of the strains, consistent with the known inability of ρ- cells to grow on galactose-containing media [15]. Thus, the ρ- stains (YL10-01, YL10-02, YL10-04 and YL10-05) show sugar and temperature sensitivity; the ρ+ strains (YL09-01 and YL10-03) do not. In summary, yeast are largely insensitive to the level of Trz1p, provided there is some minimal level of the protein; the apparent sensitivity of some strains to galactose-induced Trz1p expression is attributable to the non-specific galactose-sensitivity of ρ- yeast.

ELAC2 does not functionally substitute for TRZ1; motifs required for Trz1p function

Since TRZ1 is the yeast homolog of ELAC2, we tested the ability of ELAC2 to functionally complement deletion of TRZ1 in yeast. YL03-47 was transformed with an array of ELAC2 constructs (full length ELAC2, FLAG tagged ELAC2, N-domain ELAC2, C-domain ELAC2, and four full length ELAC2 variants found in prostate patients) (Table 4). Transformants were plated onto FOA to select against the TRZ1 expression plasmid and to determine if any construct could complement trz1. As shown in Table 4, neither wild type nor prostate cancer-associated ELAC2 mutants could substitute for wild type TRZ1 function in YL03-47. The human paralog of ELAC2, ELAC1, likewise failed to rescue trz1 (Table 4).

We next incorporated three specific mutations into the histidine motif region of TRZ1: (i) M535T, which corresponds to the missense substitution A541T in ELAC2 that is associated with human prostate cancer; (ii) H542ter, which corresponds to the frameshift 1641insG associated with human prostate cancer; and (iii) G548R, which corresponds to a ts mutant, G256R, that was isolated in the homologous yeast gene PSO2 [16] (Figure 2) (trz1 M535T, trz1 H542Ter, trz1 G548R; Table 4). Notably, in no case did the fusion of two copies of the FLAG epitope to TRZ1 alleles influence results (Table 4). The missense mutant TRZ1 allele (trz1 M535T) complements trz1 as well as the wild type gene. However, neither trz1 G548R in which a conserved glycine in the histidine motif is altered, nor the mutant TRZ1 truncated after His542 in the same motif (trz1 H542Ter) could complement trz1 in yeast.

Figure 2
figure 2

Protein multiple sequence alignment across the core histidine motif of selected ELAC/ tRNase Z family members. The ELAC/tRNase Z family members were selected from Saccharomyces cerevisiae (Sce), Schizosaccharomyces pombe (Spo), Arabidopsis thaliana (Ath), Drosophila melanogaster (Dme), Homo sapiens (Has), Escherichia coli (Eco) and Methanothermobacter thermautotrophicum (Mth). The boxed residues in the S. cerevisiae sequence are the amino acids that we mutagenized in our effort to create a ts mutant. 1, S. cerevisiae M535 corresponds to human A541. 2, Y537L is ts. 3, L538K is ts. 4, H542 corresponds to human H548, the position of a prostate cancer associated frameshift. 5, G548 corresponds to G256 of S. cerevisiae PSO2; although PSO2 G256R is a ts mutant [16], TRZ1 G548R is lethal. * denotes identical amino acid, and : /. indicates homologous conservation across species.

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Motif searches identified a putative P-loop (A/GxxxxGKS/T) at amino acids 276–283 of human ELAC2 (AxxxxGKS). This sequence element is located near the center of the N-domain and is clearly conserved among the eukaryotic orthologs. The corresponding sequence in Trz1p (AxxxxGQT, residues 224–231) falls within the range of variation for the structural P-loop recently explored by Brakoulias et al [17]. In the homology between the N- and C-domains of the protein, this candidate P-loop corresponds to a strongly conserved element near the middle of the C-domain (residues 693–700 of Trz1p), which also has features reminiscent of the structural P-loop [17]. For simplicity, we will refer to these as the first and second putative P-loops. In so doing, however, we recognize that they may be examples of some other conserved structural element. We mutated the key residues in both putative P-loops individually and tested the mutants' abilities to complement trz1. The first putative P-loop is dispensable for survival because mutants trz1 G229D and trz1 Q230A, which should destroy its function if it is a P-loop [18, 19] (and are far outside the cross-species range of variation of the sequence element if it is not a P-loop), can functionally substitute for the essential function of Trz1p. However, the second putative P-loop is required for yeast survival because mutants here (trz1 G698D and trz1 D699A) cannot complement TRZ1 deletion. Sequence analysis shows that TRZ1 may be the result of ancient tandem duplication/fusion that produced a gene with homologous amino and carboxyl halves. We therefore tested whether either the N- or C- domain of TRZ1 alone is capable of rescuing trz1. Neither half could rescue (FLAG.TRZ1.C-term and FLAG.TRZ1.N-term), even when fused to the complementary domain of ELAC2 (FLAG.TRZ1-ELAC2 and ELAC2-TRZ1) (Table 4).

Screen for and characterization of temperature sensitive (ts) TRZ1 mutants

We screened for ts mutants of Trz1p with PCR-based random mutagenesis and bacterial mutator approaches; neither of these attempts identified ts mutants. To circumvent technical difficulties, we used a targeted approach instead. We designed degenerate oligos targeting sequence encoding amino acids 535, 536, 537, 538, 541 and 548 in the Trz1p histidine motif (Figure 2). These amino acids were selected for mutagenesis because, with the exception of residue 548, they are less conserved across species than the canonical residues of the histidine motif; therefore, mutation may be less likely to completely inactivate protein function (Fig. 2). G548 was targeted because, as mentioned before, a ts mutant had been identified at the corresponding amino acid in S. cerevisiae PSO2. We generated 6 mini-libraries, each of which targets one of these residues in YL03-47, and isolated the clones that appeared at 30°C but that grew slowly or not at all at 37°C. To confirm the ts phenotype plasmids from these isolates were purified and used to transform naïve YL03-47. The screen identified two ts mutants of Trz1p, Y537L and L538K (Figure 3). We also found mutations at position 535, 536, 537, 538, 541 and 548 that are lethal at both 30°C and 37°C. Observation of lethal missense substitutions at M535 is intrinsically interesting because it is consistent with the idea that the corresponding residue A541 of human ELAC2 is under some functional constraint. More than 60% of the lethal mutations were concentrated at G548, indicating that this residue is more functionally constrained than the corresponding G256 of S. cerevisiae PSO2 (data not shown).

Figure 3
figure 3

Temperature sensitive growth of yeast expressing alleles trz1-537 and trz1-538. Isolates of yeast expressing trz1-537 (A1, B1, C1, D1) or trz1-538 (B3, C3, D3, E3) (YL03-47 with ts alleles on a LEU2 plasmid) were streaked onto SC-LEU plates and incubated at RT or 37°C. YPH499 and YL03-47 were used as controls.

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We integrated TRZ1, trz1-537 (encodes Trz1p Y537L) and trz1-538 (encodes Trz1p L538K) into the LEU2 locus in a trz1 background to yield YL13-01, YL13-02, and YL13-03, respectively. As shown in Figure 4, the ts mutants grew more slowly on glucose medium than did the controls at all temperatures tested. The inhibition of growth was more obvious at higher temperature. Growth of the ts mutants was further slowed on sucrose. When plated on raffinose, ts mutants grow only at 30°C. YL13-02 and YL13-03 are also petite mutants (i.e., ρ-) while YL13-01 is not (Table 1). The ability of YL13-01 but not YL13-02 or YL13-03 to grow on galactose can therefore be explained in terms of the known inability of ρ- yeast to grow on galactose [15]. Table 5 summarizes the doubling time for each ts mutant at different temperatures in SC-Leu medium containing glucose. At 30°C YL13-02 and YL13-03 grow at about the same rate in liquid medium containing glucose as does YL13-01. However, at 37°C the ts strains grow significantly more slowly in glucose (2–3 fold increase in doubling time) than does YL13-01.

Figure 4
figure 4

Growth at different temperatures of yeast expressing TRZ1, trz1-537 or trz1-538 alleles on media with various carbon sources added. Yeast bearing TRZ1 alleles integrated at the LEU2 locus [YL13-01 (WT), YL13-02 (tr1-537) and YL13-03 (trz1-538)] were serially diluted and spotted onto SC-LEU with different sugars added. Plates were incubated at RT (upper), 30°C (middle) and 37°C (lower).

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Table 5 Doubling time of ts allele integrants
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We integrated the trz1-537 allele at the TRZ1 locus to yield YL12-01. YL12-01 exhibits a slow-growth phenotype like that of the LEU2 integrants (YL13-02 and YL13-03). YL12-01 grows slower on glucose media compared with the isogenic wild type YPH499 at all temperatures tested and this growth difference increases at 37°C (data not shown). The trz1-537 integrants exhibit severe ts growth on sucrose containing medium and they do not grow on raffinose and galactose containing medium (data not shown). The doubling time of these mutants in glucose-supplemented liquid medium is in agreement with results from the plate assay (Table 5). To verify that the growth phenotype and sugar sensitivity observed with trz1-537 integrants do not result from a cloning artifact, we did the following. YPH499 and YL12-01 were transformed with empty vector (p416) or p416/TRZ1 and plated onto SC-Ura containing glucose. Once grown, colonies were replica-plated onto SC-Ura supplemented with glucose, sucrose, raffinose or galactose. Providing a wild type TRZ1 on a CEN vector restored the growth of YL12-01 to the level of YPH499 on all media tested (Figure 5). YL12-01 transformed with an empty vector grew more slowly than YPH499 on all media tested, with the decrease in growth rate in the order of glucose, sucrose, raffinose and galactose media, in agreement with the previous plate assay. In addition, we checked the ρ status for these transformants. Providing a copy of wild type TRZ1 on a CEN vector (YL12-02) converted YL12-01 from ρ- to ρ+ (Table 1), indicating that the petite phenotype of YL12-01 may not be due to the loss of mitochondrial DNA. Instead, it may result from the interference of important mitochondrial functions, suggesting that Trz1p also plays a role inside mitochondria. However, normal growth on a non-fermentable carbon source was not restored in diploid strains derived from crosses between ts mutant strains and a haploid ρ0 tester strain (Table 1 and data not shown). Given the fact that the tester strain should have a wild type copy of TRZ1 and ts mutant haploid strains should have mitochondrial DNA, we speculate that ts alleles of TRZ1 may have pleiotropic effects on certain important activities in mitochondria. The effect on some of these activities may be dominant, and mating with a wild type haploid strain lacking mitochondrial DNA may not fully restore the mitochondrial functions.

Figure 5
figure 5

Wild type TRZ1 restores growth at 37°C of YL12-01 on media supplemented with different sugars. YPH499 (A) and YL12-01 (B) were transformed with either an empty vector (upper plates) or a vector bearing the wild type TRZ1 gene (lower plates). Transformants were replica-plated onto SC-URA containing different sugars and incubated at 37°C for 2 days. Wild type TRZ1 restores the growth of ts strain YL12-01 on all carbon sources.

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To determine if the trz1-537 allele causes any cell cycle arrest phenotype, we analyzed YL12-01, with YPH499 as control, by FACS; both strains were grown at RT or 37°C for this study. Allele trz1-537 caused a 1 hour delay in cells entering 2N (M phase) at 37°C. When yeast reached stationary phase, YL12-01 had more cells in 2N (M phase) than YPH499 grown under the same conditions (data not shown). When viewed under a microscope the trz1-537 integrants were observed to aggregate when grown at 37°C while parental YPH499 cells did not. With YL12-01 we also observed enlarged cells and cells with elongated buds; the percentage of these abnormally shaped cells increased with the length of time that the cells were grown at 37°C (data not shown). Therefore, trz1-537 allele does not cause any cell cycle arrest phenotype and the abnormal shape we observed is most likely the result of cell death in yeast.

High copy suppressor screen

During the characterization of YL12-01, we identified a window of two days wherein it would be possible to screen for high copy suppressors of trz1-537. As shown in Figure 6, at 30°C, the appearance of YL12-01/p416Gal1 transformants takes 4 days on SC-URA. However, colonies appear after only 2 days when the same strain is transformed with p416/TRZ 1 (YL12-02). YPH499 transformed with vector or TRZ1 resulted in observable colonies after 2 days of incubation at 30°C on SC-URA. Therefore, we screened for high copy suppressors of trz1-537 by using yeast genomic fragments to transform YL12-01 and selecting transformants that had grown out in 2-3 days. We screened approximately 34,000 genomic fragments and isolated 4 suppressor clones. Two of the four clones yielded an identical sequence from a 8057 bp genomic region on chromosome 12 (253,621-261,678) that contains four genes (ERG3, YLR057w, SHM2 and REX2). One of the remaining clones includes a 2594-bp genomic sequence from chromosome 11 that contains the full-length coding region of TRZ1 plus 25 bp of 5'UTR and 53 bp of 3'UTR. The 4th clone contains chromosome 12 sequences at one end (from 794,633 down) and Ty3 LTR at the other. This last clone was not pursued further.

Figure 6
figure 6

Growth difference exploited for suppressor screen. YPH499 and YL12-01 were transformed with either an empty vector (p416) or with p416/TRZ1 expressing a wild type TRZ1 gene. Transformants were plated onto SC-URA and incubated at 30°C for 3 days.

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We cloned each of the four genes on the 8057 bp region of chromosome 12 and inserted these independently into CEN and 2 μ expression vectors containing the CYC1 promoter. We tested each gene for an ability to rescue the ts phenotype of trz1-537 yeast. Only REX2 rescued the ts phenotype and rescue was observed with both CEN and 2 μ vectors. REX2 encodes a RNA 3' processing enzyme that localizes to mitochondria [20, 21].

Discussion

In this paper, we report investigation of TRZ1, the yeast homolog of ELAC2. Altered expression of TRZ1 in yeast does not cause any altered growth phenotype. Conservation of the histidine motif and the C-domain putative P-loop is important for the essential function of Trz1p in yeast. Trz1p (Y537L), a ts mutant, exhibits a slow growth phenotype on a variety of yeast media. Additional copies of REX2 rescue the slow growth phenotype associated with Trz1p (Y537L), providing genetic confirmation that the product of TRZ1 plays a role in RNA processing.

1. Altered expression of TRZ1 is not detrimental to yeast growth

Although TRZ1 is an essential gene, yeast can tolerate altered expression of TRZ1. We down-regulated TRZ1 message by deleting the endogenous gene and expressing TRZ1 from a Gal1 promoter on a CEN plasmid. We could not detect any apparent growth defect when TRZ1 expression was suppressed on glucose-containing medium. We also over-expressed exogenous TRZ1 message and protein from a Gal1 promoter on backgrounds of either an intact or deleted endogenous TRZ1 locus. In neither of these backgrounds does over-expression of TRZ1 cause any significant alteration of yeast doubling time. We also attempted to decrease TRZ1 mRNA by integration of a thermal labile cassette (Degron approach; data not shown) or by insertion of a Gal1 promoter just upstream of the endogenous TRZ1 coding sequence. In these attempts, however, we did not obtain any integrants that were not petite mutants.

In conclusion, under or overexpression of TRZ1 does not grossly affect growth or cell cycle; however, the tendency for alteration in this gene to cause a petite phenotype confounds our ability to detect small perturbations.

2. ELAC2 cannot substitute for TRZ1 function and the C-domain putative P-loop is important for TRZ1 essential function

Human ELAC2 cannot rescue TRZ1 deletion in yeast. We observed this with both full-length ELAC2 and with TRZ1/ELAC2 and ELAC2/TRZ1 fusions. The same lack of complementation was observed with ELAC1, the paralog of ELAC2. These results could be explained if ELAC1 and ELAC2 have overlapping but not totally redundant functions. TRZ1 is more similar to ELAC2 than to ELAC1, but Trz1p may accomplish functions in yeast performed by both ELAC1 and ELAC2 in humans. Therefore, substituting TRZ1 with either one of these genes may not replace all of the essential functions of Trz1p in yeast. It would be interesting to see whether supplying both human ELAC1 and ELAC2 could rescue TRZ1 deletion in yeast.

A motif search found two putative nucleotide-binding motifs (P-loops) in TRZ1 and ELAC2, one in the N-domain and one in the C-domain of each gene [2]. The finding that yeast tolerates mutation of the N-domain but not the C-domain P-loop of TRZ1 suggests the putative C-domain nucleotide-binding motif plays an important role in Trz1p function. The fact that the C-domains of TRZ1 and ELAC2 contain the most conserved sequence across other organisms also supports the notion that the vital functions of TRZ1 require that this sequence element is intact. However, the N-domain of Trz1p is also necessary for function because expression of an N-terminally deleted TRZ1 does not rescue trz1 yeast, nor did the expression of the fusion protein between the N-domain of human ELAC2 and the C-domain of yeast TRZ1. This observation is consistent with the results from Nashimoto's lab [9]. Their data suggest that the N-terminal half of ELAC2 (human tRNase ZL), to which family yeast TRZ1 also belongs, is responsible for substrate specificity and confers RNase 65 activity that is not present in ELAC1 (human tRNase ZS). And the activities of tRNase Z and RNase 65 toward a set of substrates between human ELAC2 and yeast TRZ1 are different, suggesting the N-domain of tRNase ZLs is essential for certain functions in the cell and they may not functionally equivalent among different species [9].

3. Potential role of TRZ1 in rRNA biogenesis, mRNA splicing and mitochondrion maintenance in addition to its role in tRNA processing

The majority of the RNA in growing yeast is noncoding [13] including rRNA, tRNA, snRNA, snoRNA as well as the RNA components of RNase P and RNase MRP. These RNAs are involved in processes that are vital to cell survival. The biogenesis of rRNA is a complicated process that involves a large number of proteins and RNAs that catalyze or modulate transcription, translocation, and various processing reactions, including exonucleolytic and endonucleolytic cleavages, ligations, terminal additions (5S), and nucleoside modifications. The details of yeast rRNA processing and some of the complexes involved have been described [13, 14, 22, 23].

REX proteins (R NA ex onucleases) belong to a family of well-conserved RNA 3' → 5' exonucleases [24]. Rex1p is required for 5S and tRNA-Arg3 maturation. Deletion of Rex2p results in a defect in the processing of the U4 snRNA that is involved in mRNA splicing. Rex3p is required for the maturation of MRP RNA, an important endonucleolytic complex involved in processing both rRNA and tRNA. Rex1p and Rex2p have been shown to be involved in the final maturation of 5.8S rRNA. Rex1p, Rex2p and Rex3p are also involved in the maturation of U5 snRNA and the RNA subunit of RNase P, the RNase that is involved in both tRNA 5' and rRNA maturation. In addition to participating in tRNA and rRNA processing, Rex2p has been implicated in spliceosome assembly by association with the snRNP U2 component PRP11, in DNA repair by association with Rad50p, and in cytoskeleton reorganization by association with the actin cortical patch component Cof1p [25]. Rex2p localizes to the mitochondria and interacts genetically with YME1 and YME2, components of the inner mitochondrial membrane that participate in mitochondrion organization and biogenesis [20, 21]. In summary, the REX proteins function in both RNA maturation and mitochondrial maintenance. The finding that REX2 rescues the slow growth phenotype of yeast expressing Trz1p (Y537L), suggests that TRZ1 may also participate in those same processes. Further support for this hypothesis comes from the finding that TRZ1 exists in a complex with NUC1 [26, 27], an RNase and DNA endo/exonuclease that localizes to the mitochondria and is involved in mitochondrial DNA recombination. In addition, we observed that supplying an extra copy of wild type TRZ1 on a CEN plasmid alters YL12-01, converting the ts mutant Trz1p (Y537L) from ρ- to ρ+ (Table 1). The mitochondrial localization of Trz1p [26] together with the suppression of the Trz1p (Y537L) phenotype by REX2 and the effect of expression of wt TRZ1 on the petite mutant status of YL12-01 suggest that Trz1p may participate in the activities of mitochondria.

The human homolog of TRZ1, ELAC2, was identified as a candidate prostate cancer susceptibility gene [2]. Schiffer et al. [5] purified a nuclear tRNase Z from wheat germ and found two homologs of tRNase Z in A. thaliana, NUZ (nuclear RNase Z) and CPZ (chloroplast RNase Z). Both Arabidopsis homologs have tRNase Z activity in vitro. These authors also showed that NUZ is a homolog of ELAC1 and represents a family of proteins that is conserved from bacteria to humans. Subsequently, Takaku et al. showed that human ELAC2, its paralog ELAC1, and the yeast homolog TRZ1 have tRNase Z activity in vitro [3]. The finding in this study that extra copies of REX2 rescue a ts allele of TRZ1 confirms a role for Trz1p in the processing of RNA, placing TRZ1 in particular in rRNA and tRNA maturation pathways. This is consistent with previous results. First, rRNA biogenesis involves processes both in the nucleolus and cytoplasm, and TRZ1 protein localizes to both of these cellular compartments [28]. Second, many proteins and some complexes, for example, MRP, RNase P and the RNA polymerase III complex, participate both in tRNA and rRNA processing and synthesis. Therefore it is possible that TRZ1 participates in both tRNA and rRNA processing. Third, using a tet-promoter TRZ1 allele and a custom DNA oligo array for noncoding RNA biogenesis, Peng et al. demonstrated that TRZ1 has an undefined role in the processing of 35S rRNA [29]. The high-copy suppressor result could also be explained more straightforwardly if we assume that Trz1p (Y357L) has diminished or abolished tRNase Z activity, which could result in low level of mature tRNAs, and Rex2p could remove the 3' trailers from pre-tRNAs exonucleolytically, that could result in restored mature tRNA levels.

Since ELAC2 cannot functionally complement TRZ1 deletion, we cannot directly test in yeast the functional effect of the human ELAC2 missense substitution A541T, which appears to confer modest risk of prostate cancer [30]. Although this variant does not seem to affect the tRNase Z activity of human ELAC2 in vitro [3], we did find that some missense substitutions at the corresponding yeast residue, M535, are lethal, demonstrating that this position is under functional constraint. Recent demonstrations that hoe-1, the C. elegans homolog of ELAC2, plays a role in germline proliferation [31], and human ELAC2 maybe involved in cell cycle regulation [4], may help better illuminate the contribution of ELAC2 to the etiology of human prostate cancer.

Conclusion

We have undertaken a genetic study of yeast TRZ1 gene, the homolog of prostate cancer susceptibility gene ELAC2. Our data suggest that the absolute level of TRZ1 transcript is not important for the fitness of yeast, the vital functions of TRZ1 require an intact C-terminal P-loop and some conserved residues in Histidine motif. More importantly, our high copy suppressor screen suggests that TRZ1 is involved in RNA processing, especially rRNA processing and mitochondrial biogenesis.

Methods

Strains and Reagents

The strains used in this study are listed in Table 1. The petite status of each strain, determined by growth on a non-fermentable carbon source, is listed in Table 1. YPH499, YPH500, and YPH501 were purchased from Strategene (La Jolla, CA). Genetic manipulations, yeast growth conditions and preparation of yeast media and plates were as described [32]. Yeast transformation and gene disruption were carried out using lithium acetate [32]. Cloning and manipulation of yeast plasmids were done in bacterial strain DH5α. Yeast medium was purchased from Qbiogene (Carlsbad, CA). 5'-Fluoroorotic acid (5'-FOA), used at a concentration of 0.1%, was purchased from Toronto Research Chemicals, Inc. (Ontario, Canada).

Plasmid and strain construction

A 3.7 kb fragment containing TRZ1 coding sequence and intergenic regions was amplified from yeast genomic DNA (primer sequences available upon request) and cloned into pBluescript-KS (BSKS/TRZ1). To express epitope-tagged protein, TRZ1 N- and C-termini were re-engineered using PCR. We generated BSSK TRZ1•FLAG (2 copies of a FLAG epitope fused to the C-terminus), BSSK/TRZ1•V5 (1 copy of a V5 epitope fused to the C-terminus), BSSK/TRZ1•3XHA or BSSK/TRZ1•3Xmyc (3 copies of HA or Myc epitopes fused to the C-terminus, respectively) and BSSK/FLAG•TRZ1 (2 copies of a FLAG epitope fused to the N-terminus). Site-directed mutagenesis was performed with QuikChange Site-Directed Mutagenesis kit from Stratagene (La Jolla, CA) following their protocols.

YL03-47 is a trz1 haploid strain in which the genomic trz1 is complemented by high-copy episomal TRZ1. To construct this strain, first a TRZ1/trz1 heterozygote, was generated using a DNA fragment (TRZ1•hisG•URA3•hisG•TRZ1) that contained in succession: the TRZ1 promoter region; the 5' 37 codons of TRZ1; URA3 flanked by hisG cassettes; the 3' 123 codons of TRZ1; and 108 bp of sequence 3' of the TRZ1 stop codon. YPH501 was transformed with this fragment, and TRZ1/trz1 heterozygotes were selected as uracil prototrophs. Next, one such TRZ1/trz1 heterozygote was plated onto FOA to select a URA3-deficient derivitive of the TRZ1/trz1 heterozygote. This TRZ1/trz1 uracil auxotroph was then transformed with a 2 μ URA3 TRZ1 expression plasmid (pRS426/TRZ1). Finally, to obtain YL03-47, tetrads from the resulting transformants were dissected, and URA+ colonies were screened by PCR for the presence of hisG sequence at the endogenous TRZ1 locus.

YL12-01, in which the chromosomal TRZ1 is replaced with trz1-537 allele, is constructed from YPH499 following a two-step gene replacement strategy. DNA fragment encoding the ts mutant trz1-537 allele was cloned into an URA3-marked integrating vector (p406/trz1-537). The resulting plasmid was linearized with Bgl II and transformed into YPH499 to integrate into TRZ1 chromosomal locus. Uracil prototrophs were selected from the transformation and plated on FOA to select cells that had undergone excision of the plasmid. The replacement of wild type TRZ1 with the ts mutant trz1-537 allele was confirmed with sequencing.

YL13-01, YL13-02 and YL13-03, in which the chromosomal TRZ1 is deleted and a wild type TRZ1 or a trz1-537 allele or a trz1-538 allele is integrated at the LEU2 locus, were generated as follows. DNA fragments encoding TRZ1, or the ts mutant alleles trz1-537 or trz1-538 were cloned into an integrating LEU2-marked vector (p405/TRZ1). The resultant plasmids were linearized with BstXI, and YL03-47 was transformed with the fragments for integration into the leu2-Δ 1 locus. Leucine prototrophs were selected from the transformation and plated on FOA to select cells that had lost pRS426/TRZ1. The identity of the integrated alleles was confirmed by PCR and sequencing.

Western blot

Yeast cells were grown as indicated in the text. Usually, about 1.5 ml yeast culture with an A600 of about 0.6 was centrifuged and the pellet resuspended in 150 μL boiling buffer (50 mM Tris pH8.0, 0.1% Bromophenol blue, 2% SDS, 10% glycerol and fresh 0.1 M DTT). The yeast suspension was heated to 95°C for 20 minutes followed by vortexing and centrifugation at 14000 rpm for 1 minute. The resulting cellular extract was loaded onto a SDS-PAGE gel. Electrophoresis, transfer and Western blot were carried out using Invitrogen's XCell SureLock system following their protocol. Anti-myc and anti-V5 antibodies were purchased from Invitrogen (Calsbad, CA) and used at 1:2000 dilution. Anti-FLAG antibody was purchased from Sigma (St. Louis, MO) and used at 1:400 dilution.

Temperature-sensitive (ts) mutant screen

To prepare for a screen for TRZ1 mutations that rendered yeast growth temperature-sensitive, the codons of specific TRZ1 amino acids were mutagenized. We targeted amino acids 535, 536, 537, 538, and 541 for ts screening. To mutagenize amino acid 535, a pair of oligos with degenerate nucleotides encoding residue 535 were synthesized. The primer sequences were as following: 079c.535.F (5'-CAG GAT TTG AAA NNN ATA TAT CTG AGT CAC-3'), 079c.535.R (5'-ACT CAG ATA TAT NNN TTT CAA ATC CTG AAA TAT TG-3'), 079c.NdeI (5'-GAT TAT GAT TGT GCT GAG CTT GGC-3'), 079c.BglII (5'-TTC TTC ACG GCA TCC TCC AGT AGC-3'). Two fragments were generated by primary PCR with wild type TRZ1 coding sequence as template and 079c.NdeI/079c.535.R and 079c.535.F/079c.BglII as primers. About 100 ng of each of the resulting fragments were mixed and used as templates for secondary PCR using 079c.NdeI/079c.BglII as primer. The resulting PCR products were purified by Qiagen PCR column, mixed with p415/TRZ1 (Δ NdeI-NcoI) that lacks TRZ1 sequence between Nde I and NcoI sites, and the mixture used to transform competent YL03-47. Transformants were plated onto SC-LEU. For other targeted amino acids, PCR-generated mini-libraries were made by following a similar strategy to that outlined above. About 500 colonies were screened for each targeted amino acid. After two days' growth on SC-Leu media at 30°C, colonies were replica-plated onto two SC-Leu+FOA plates, one of which was incubated at 30°C, while the other was incubated at 37°C. After 24 hours colonies that grew at 30°C but failed to grow at 37°C were isolated from the original SC-LEU plate. The LEU2 marked plasmids were isolated, sequenced, and used to retransform YL03-47. Transformants were plated on FOA to select yeast that had lost p426/TRZ1 and streaked onto SC-LEU+FOA plates to confirm the temperature sensitive phenotype.

High copy suppressor screen

A 2 μ URA3-marked yeast genomic library (gift from Mike Forte and Beth Lachlyd, Vollum Institute, Portland, OR) was used to transform YL12-01. Transformants were plated onto SC-URA and after four days' incubation at 30°C, colonies were picked. About 90 colonies were picked out of a total of 70,000 colonies screened. The library plasmids were isolated and used to retransform YL12-01. The suppressor phenotype was confirmed by comparing the growth of YL12-01 transformed with each library plasmid with that of YL12-01 transformed with p416/Gal1 and p416/TRZ1. The sequence of the high copy suppressors was determined.

Abbreviations

TRZ1 :

tRNase Z 1

tRNase Z:

transfer RNA (tRNA) 3' processing endoribonuclease

CTD:

C-terminal domain

REX:

RNA exonuclease

tRNA:

transfer RNA

rRNA:

ribosomal RNA

snRNA:

small nuclear RNA

snoRNA:

small nucleolar RNA

References

  1. Ferlay J, Whelan SL: Cancer Incidence in Five Continents. Edited by: Parkin DM, Whelan SL, Feraly J, Teppo L, Thomas DB. 2002, VIII: 91-92. and 208–210, International Agency for Research on Cancer, Lyon, France

    Google Scholar 

  2. Tavtigian S, Simard J, Teng DHF, Abtin V, Baumgard M, Beck A, Camp NJ, Carillo AR, Chen Y, Dayananth P, Desrochers M, Dumont M, Farnham JM, Frank D, Frye C, Ghaffari S, Gupte J, Hu R, Iliev D, Janecki T, Kort EN, Laity KE, Leavitt A, Leblanc G, McArthur-Morrison J, Pederson A, Penn B, Peterson K, Reid JE, Richards S, Schroeder M, Smith R, Snyder SC, Swedlund B, Swensen J, Thomas A, Tranchant M, Woodland A, Labrie F, Skolnick MH, Neuhausen S, Rommens J, Cannon-albright LA: A candidate prostate cancer susceptibility gene at chromosome 17p. Nat Genet. 2001, 27: 172-180. 10.1038/84808

    Article  CAS  PubMed  Google Scholar 

  3. Takaku H, Minagawa A, Takagi M, Nashimoto M: A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease. Nucleic Acids Res. 2003, 31: 2272-2278. 10.1093/nar/gkg337

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Korver W, Guevara C, Chen Y, Neuteboom S, Bookstein R, Tavtigian S, Lees E: The product of the candidate prostate cancer susceptibility gene ELAC2 interacts with the γ-tubulin complex. Int J Cancer. 2003, 104: 283-288. 10.1002/ijc.10945

    Article  CAS  PubMed  Google Scholar 

  5. Schiffer S, Rösch S, Marchfelder A: Assigning a function to a conserved group of proteins: the tRNA 3'-processing enzymes. EMBO J. 2002, 21: 2769-2777. 10.1093/emboj/21.11.2769

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Pellegrini O, Nezzar J, Marchfelder A, Putzer H, Condon C: Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis. EMBO J. 2003, 22: 4534-4543. 10.1093/emboj/cdg435

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Dubrovsky EB, Dubrovskaya VA, Levinger L, Schiffer S, Marchfelder A: Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3' ends in vivo. Nucleic Acids Res. 2004, 32: 255-262. 10.1093/nar/gkh182

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Minagawa A, Takaku H, Takagi M, Nashimoto M: A Novel Endonucleolytic Mechanism to Generate the CCA 3' Termini of tRNA Molecules in Thermotoga maritime. J Biol Chem. 2004, 279: 15688-15697. 10.1074/jbc.M313951200

    Article  CAS  PubMed  Google Scholar 

  9. Takaku H, Minagawa A, Takagi M, Nashimoto A: The N-terminal half-domain of the long form of tRNase Z is required for the RNase 65 activity. Nucleic Acids Res. 2004, 32: 4429-4438. 10.1093/nar/gkh774

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Herbert A, Rich A: RNA processing and the evolution of eukaryotes. Nat Genet. 1999, 21: 265-269. 10.1038/6780

    Article  CAS  PubMed  Google Scholar 

  11. Xiao S, Houser-Scott F, Engelke DR: increased complexity to cope with the nuclear pre-tRNA pathway. J Cell Physiol. 2001, 187: 11-21. 10.1002/1097-4652(200104)187:1<11::AID-JCP1055>3.0.CO;2-K

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Yoo CJ, Wolin SL: The yeast La protein is required for the 3' endonucleolytic cleavage that matures tRNA precursors. Cell. 1997, 89: 393-402. 10.1016/S0092-8674(00)80220-2

    Article  CAS  PubMed  Google Scholar 

  13. Perry RP: RNA processing comes of age. J Cell Biol. 1981, 91: 28s-38s. 10.1083/jcb.91.3.28s

    Article  CAS  PubMed  Google Scholar 

  14. Kressler D, Linder P, de La Cruz J: Protein trans-acting factors involved in ribosome biogenesis in Saccharomyces cerevisiae. Mol Cell Biol. 1999, 19: 7897-912.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Carlson M, Osmond BC, Botstein D: Mutants of yeast defective in sucrose utilization. Genetics. 1981, 98: 25-40.

    PubMed Central  CAS  PubMed  Google Scholar 

  16. Niegmann E, Brendel M: A single amino acid change in SNM1 -ecoded protein leads to thermoconditional deficiency for DNA cross-link repair in Saccharomyces cerevisiae. Mutat Res. 1994, 315: 275-279.

    Article  Google Scholar 

  17. Brakoulias A, Jackson RM: Towards a structural classification of phosphate binding sites in protein-nucleotide complexes: an automated all-against-all structural comparison using geometric matching. Proteins. 2004, 56: 250-260. 10.1002/prot.20123

    Article  CAS  PubMed  Google Scholar 

  18. Wu TH, Marinus MG: Dominant negative mutator mutations in the mutS gene of Escherichia coli. J Bacteriol. 1994, 176: 5393-5400.

    PubMed Central  CAS  PubMed  Google Scholar 

  19. Shen H, Yao B, Mueller DM: Primary structural constraints of P-loop of mitochondrial F1-ATPase from yeast. J Biol Chem. 1994, 269: 9424-9428.

    CAS  PubMed  Google Scholar 

  20. Hanekamp T, Thorsness PE: Inactivation of YME2/RNA12, which encodes an integral inner mitochondrial membrane protein, causes increased escape of DNA from mitochondria to the nucleus in Saccharomyces cerevisiae. Mol Cell Biol. 1996, 16: 2764-2771.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Hanekamp T, Thorsness PE: YNT20, a bypass suppressor of yme1 yme2, encodes a putative 3'-5' exonuclease localized in mitochondria of Saccharomyces cerevisiae. Curr Genet. 1999, 34: 438-48. 10.1007/s002940050418

    Article  CAS  PubMed  Google Scholar 

  22. Lewis JD, Tollervey D: Like attracts like: getting RNA processing toether in the nucleus. Science. 2000, 288: 1385-1389. 10.1126/science.288.5470.1385

    Article  CAS  PubMed  Google Scholar 

  23. Faber AW, Dijk MV, Raue HA, Vos JC: Ngl2p is a Ccr4p-like RNA nuclease essential for the final step in 3'-end processing of 5.8S rRNA in Saccharomyces cerevisiae. RNA. 2002, 8: 1095-1101. 10.1017/S1355838202021027

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. van Hoof A, Lennertz P, Parker R: Three conserved members of the RNase D family have unique and overlapping functions in the processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs in yeast. EMBO J. 2000, 19: 1357-1365. 10.1093/emboj/19.6.1357

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  25. Ho Y, Gruhler A, Heilbut A, Bader GD, Moore L, Adams SL, Millar A, Taylor P, Bennett K, Boutilier K, Yang L, Wolting C, Donaldson I, Schandorff S, Shewnarane J, Vo M, Taggart J, Goudreault M, Muskat B, Alfarano C, Dewar D, Lin Z, Michalickova K, Willems AR, Sassi H, Nielsen PA, Rasmussen KJ, Andersen JR, Johansen LE, Hansen LH, Jespersen H, Podtelejnikov A, Nielsen E, Crawford J, Poulsen V, Sorensen BD, Matthiesen J, Hendrickson RC, Gleeson F, Pawson T, Moran MF, Durocher D, Mann M, Hogue CW, Figeys D, Tyers M: Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature. 2002, 415: 180-183. 10.1038/415180a

    Article  CAS  PubMed  Google Scholar 

  26. Hazbun TR, Malmström L, Anderson S, Graczyk BJ, Fox B, Riffle M, Sundin BA, Aranda JD, McDonald WH, Chiu C, Snydsman BE, Bradley P, Muller EGD, Fields S, Baker D, Yates JR, Davis TN: Assigning function to yeast proteins by integration of technologies. Mol Cell. 2003, 12: 1353-1365. 10.1016/S1097-2765(03)00476-3

    Article  CAS  PubMed  Google Scholar 

  27. Gavin AC, Bösche M, Krause R, Grandi P, Marzioch M, Bauer A, Schultz J, Rick JM, Michon AM, Cruciat CM, Remor M, Höfert C, Schelder M, Brajenovic M, Ruffner H, Merino A, Klein K, Hudak M, Dickson D, Rudi T, Gnau V, Bauch A, Bastuck S, Huhse B, Leutwein C, Heurtier MA, Copley RR, Edelmann A, Querfurth E, Rybin V, Drewes G, Raida M, Bouwmeester T, Bork P, Seraphin B, Kuster B, Neubauer G, Superti-Furga G: Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature. 2002, 415: 141-147. 10.1038/415141a

    Article  CAS  PubMed  Google Scholar 

  28. Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O'Shea EK: Global analysis of protein localization in budding yeast. Nature. 2003, 425: 686-691. 10.1038/nature02026

    Article  CAS  PubMed  Google Scholar 

  29. Peng WT, Robinson MD, Mnaimneh S, Krogan NJ, Cagney G, Morris Q, Davierwala AP, Grigull J, Yang X, Zhang W, Mitsakakis N, Ryan OW, Datta N, Jojic V, Pal C, Canadien V, Richards D, Beattie B, Wu LF, Altschuler SJ, Roweis S, Frey BJ, Emili A, Greenblatt JF, Hughes TR: A panoramic view of yeast noncoding RNA processing. Cell. 2003, 113: 919-933. 10.1016/S0092-8674(03)00466-5

    Article  CAS  PubMed  Google Scholar 

  30. Camp NJ, Tavtigian SV: Meta-analysis of associations of the Ser217Leu and Ala541Thr variants in ELAC2 (HPC2) and prostate cancer. Am J Hum Genet. 2002, 71: 1475-1478. 10.1086/344516

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  31. Smith M, Levitan DJ: The Caenorhabditis elegans homolog of the putative prostate cancer susceptibility gene ELAC2, hoe-1, plays a role in germline proliferation. Dev Biol. 2004, 266: 151-160. 10.1016/j.ydbio.2003.10.016

    Article  CAS  PubMed  Google Scholar 

  32. Guthrie C, Fink GR: Guide to Yeast Genetics and Molecular Biology, in Method in Enzymology. 1991, 194: San Diego, Academic Press, Inc

    Google Scholar 

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Acknowledgements

We thank Dr. Mike Forte and Dr. Beth Lachlyd (Vollum Institute) for sharing their high copy genomic yeast library. We thank Dr. John Manfredi for his intellectual input. We are especially grateful to Dr. Christine Klein for her critical reading and editing of the manuscript.

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Authors and Affiliations

  1. Myriad Genetics, Inc, 320 Wakara Way, Salt Lake City, UT, 84108, USA

    Yang Chen, Audrey Beck, Christina Davenport, Yuan Chen, Donna Shattuck & Sean V Tavtigian

  2. International Agency for Research on Cancer, World Health Organization, 150 Cours Albert-Thomas, 69372, Lyon Cedex, France

    Sean V Tavtigian

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Correspondence to Yang Chen.

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Authors' contributions

YC (Yang Chen) designed most of the experiments, carried out most of the genetic studies, interpreted the data and wrote the manuscript. AB carried out most of the high copy suppressor screens and ts screens. CD carried out most of the growth assays and confirmation of high copy suppressors. YC (Yuan Chen) carried out some of the ts screen and growth assays. DS and SVT have made substantial contributions to conception and design of the experiment and drafting of the manuscript.

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Chen, Y., Beck, A., Davenport, C. et al. Characterization of TRZ1, a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z. BMC Molecular Biol 6, 12 (2005). https://doi.org/10.1186/1471-2199-6-12

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