Table 1

Quality assessment of the retrieved data. Quality assessment of the retrieved data. For the 2-ΔΔCT results the table shows inclination and R2 for ΔCT plotted against log RNA concentration and inclinations and R2 for CT plotted against log RNA concentration for eEF1α and LsTryp1. For the DART-PCR results the table shows PCR-efficiencies for eEF1α and LsTryp1 calculated by DART-PCR and the p-value (one-way ANOVA) for the hypothesis that there is no difference between the efficiencies.

2-ΔΔCT results

DART-PCR results


ΔCT vs. log [RNA] (R2)

eEF1α CT vs. log [RNA] (R2)

LsTryp1 CT vs. log [RNA] (R2)

eEF1α

LsTryp1

ANOVA


Unstarved

-0.080 (0.16)

-2.7664 (0.95)

-2.7949 (0.99)

0.901

0.902

0.9863

Starved

0.065 (0.06)

-2.8815 (0.99)

-2.8171 (0.93)

0.839

0.491

0.0004

Refed

-0.052 (0.07)

-3.3109 (0.99)

-3.3845 (0.99)

0.883

1.095

0.0004


Skern et al. BMC Molecular Biology 2005 6:10   doi:10.1186/1471-2199-6-10

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