Figure 9.

CHIR99021 does not affect FOXO-1 transactivation potential. H4IIE cells were transfected with BP-1WT along with pEBG2T or pEBG2T-FOXO-1. Cells were incubated with 10 nM insulin or 2 μM CHIR99021 for 24 h prior to lysis and luciferase assays as described in materials and methods. Results are presented as % luciferase activity relative to basal (serum free) luciferase expression and are the means ± S.E. of two experiments performed in triplicate. NS; not significant.