Table 2

Nonsense mutations in SUP45 lead to decreased ascospore viability

D1628 transformed with plasmids:

Plasmid stability %

Total number of tetrads

Tetrads with indicated number of viable spores

% of His+Ura+ segregants a


4

3

2

1


pRS316/SUP45

71

49

7

19

22

1

27

pRS316/sup45-101

74

52

6

6

38

2

15

pRS316/sup45-102

71

30

1

1

26

2

5

pRS316/sup45-104

74

48

0

2

42

4

2

pRS316/sup45-105

72

35

0

0

32

3

0

pRS316/sup45-107

69

31

3

5

21

2

14


a The amount of His+Ura+ segregants is a characteristic of sup45-n allele presence (His+ is the marker for the SUP45 gene disruption and Ura+- for the pRS316 plasmid presence). The data correspond to the sum of two experiments but the cell number in nonviable colonies was calculated from only one (Table 3).

Moskalenko et al. BMC Molecular Biology 2003 4:2   doi:10.1186/1471-2199-4-2

Open Data