Table 1

The efficiency of maintenance of pRS315/SUP45 plasmid on SUP45::HIS3 [sup45-n] background

Transformants of 1A-D1628 bearing plasmids a:

% of Ura+ Leu- colonies after rounds of selection:



pRS315 [LEU2] with:

pRS316 [URA3] with:

I b

II c


SUP45

SUP45

9.5 ± 1.1

11.0 ± 0.7

SUP45

sup45-101

6.3 ± 0.8

10.9 ± 1.1

SUP45

sup45-102

2.2 ± 0.7

4.3 ± 0.9

SUP45

sup45-104

2.5 ± 0.9

12.1 ± 2.2

SUP45

sup45-105

0.3 ± 0.2

15.6 ± 1.7

SUP45

sup45-107

2.0 ± 0.3

5.0 ± 0.6


a Strain 1A-D1628 bearing disruption of the SUP45 gene (SUP45::HIS3) and the SUP45 gene on pRS315 plasmid was transformed with pRS316/sup45-n plasmids. b The frequency of pRS315/SUP45 loss in cotransformants was determined (I). c Transformants bearing only sup45-n plasmids were selected on SC- Ura medium, re-transformed with pRS315/SUP45 plasmid and the frequency of pRS315/SUP45 loss in cotransformants was determined again (II). At least 3 independent transformants were tested for each plasmid combinations. The average data with standard errors (± SE) are presented.

Moskalenko et al. BMC Molecular Biology 2003 4:2   doi:10.1186/1471-2199-4-2

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