Figure 6.

Immunofluorescence-based detection of the LDLR protein in the p3.7LDL-transfected and the native CHO-ldlA7 cells. Supernatant of IgG2b monoclonal antibody (CRL-1691, ATCC) against hLDLR was used as the primary and anti-sheep FITC conjugated as the secondary antibody. Samples were treated with SlowFade™ Light Antifade reagent and viewed under a fluorescence microscope. In transfected cells, the fluorescent signal is detectable in the cytoplasm generally and in the Golgi complex particularly. A weak background signal is visible in the native cell population