Figure 1.

Expression and purification of *VLF-1. (A) Expression of *VLF-1 in infected Sf9 cells analyzed by SDS-12% PAGE followed by Western blotting with the monoclonal antibody HA.11. Lane 1, 48 h p.i.; lane 2, 72 h p.i.. (B) Purified *VLF-1 analyzed by SDS-10% PAGE followed by staining with Coomassie brilliant blue. Lane 1, *VLF-1, 100 ng; lane M, 10-kDa protein ladder. The molecular masses of protein markers (in kilodaltons) are shown on the left.